Kure S, Tada K, Wada J, Yoshie O
Virus Res. 1986 Sep;5(4):377-90. doi: 10.1016/0168-1702(86)90030-4.
The inhibitory effects of pure recombinant human interferons alpha A and gamma (reIFN-alpha A and -gamma) on Epstein-Barr virus (EBV) infection of a human EBV-negative B cell line, BJAB, and of normal adult B lymphocytes were studied. With pretreatment for 24 h, both types of reIFNs were effective in suppressing the production of EBV specific nuclear antigen (EBNA-1) in BJAB cells 24 h after EBV-infection, as determined by the immunoblotting technique. ReIFN-alpha A was, however, a much more potent inhibitor than reIFN-gamma. With treatment starting 1 h after EBV infection, both types of reIFNs were less effective in the suppression of EBNA production. Neither of the reIFNs showed any inhibitory effect on EBNA production in the latently EBV-infected cell lines, Raji and Daudi. These results suggest that reIFNs act in the early phase of EBV infection. Both types of reIFNs were also effective in inhibiting EBV infection of normal adult B lymphocytes as demonstrated by a reduction both in [3H]thymidine incorporation 6 days after EBV infection and in the total number of proliferating cells 21 days after EBV infection. Again, reIFN-alpha A showed a greater inhibitory effect than reIFN-gamma. We also showed that in BJAB cells, reIFN-alpha A strongly induced (2'-5')oligoadenylate synthetase activity, whereas reIFN-gamma increased the surface expression of HLA class I antigens.
研究了纯重组人αA干扰素和γ干扰素（reIFN-αA和-reIFN-γ）对人EBV阴性B细胞系BJAB以及正常成人B淋巴细胞的爱泼斯坦-巴尔病毒（EBV）感染的抑制作用。经24小时预处理后，通过免疫印迹技术测定，两种类型的reIFN均可有效抑制EBV感染后24小时BJAB细胞中EBV特异性核抗原（EBNA-1）的产生。然而，reIFN-αA是比reIFN-γ更有效的抑制剂。在EBV感染后1小时开始治疗，两种类型的reIFN在抑制EBNA产生方面效果较差。两种reIFN对潜伏性EBV感染的细胞系Raji和Daudi中的EBNA产生均未显示出任何抑制作用。这些结果表明，reIFN在EBV感染的早期阶段起作用。如EBV感染后6天[3H]胸苷掺入量的减少以及EBV感染后21天增殖细胞总数的减少所证明，两种类型的reIFN在抑制正常成人B淋巴细胞的EBV感染方面也有效。同样，reIFN-αA比reIFN-γ显示出更大的抑制作用。我们还表明，在BJAB细胞中，reIFN-αA强烈诱导（2'-5'）寡腺苷酸合成酶活性，而reIFN-γ增加HLA I类抗原的表面表达。
