HANNAY C L
J Biophys Biochem Cytol. 1961 Feb;9(2):285-98. doi: 10.1083/jcb.9.2.285.
Fowler's bacillus is one of several organisms which form a non-viable inclusion or parasporal body during the process of sporulation. This body is globular and may be as large as or larger than the spore. Its position in the cell is not random; the spore is terminal and the body paracentral, lying between the spore and the remaining vegetative cell chromatin bodies. On completion of sporulation both spore and body are contained within an exosporium. The sequence in the development of the cell structures was followed in ultrathin sections of material fixed in permanganate. When sporulation is well advanced the body begins to grow from a single crystal, then presumably as a result of some disorientation in the growth process it develops as a multicrystalline body with the lattices orientated at different angles. When the body approximates the spore in size, a lamella coat is formed and an exosporium develops which eventually encircles the body and the spore. Other lamella systems microscopically similar to those surrounding the parasporal body develop free in the cytoplasm outside the exosporium. In both of these systems the number of lamellae is variable. The spore coat of Fowler's bacillus, consisting of an outer lamella layer and an inner unresolved amorphous layer has been found microscopically identical to the spore coat of B. cereus. In both organisms the lamella layer of the spore coat consists, in contrast to the other lamella systems, of a regular number of lamellae. Physiological tests would indicate that Fowler's bacillus is a variety of B. cereus.
福勒氏芽孢杆菌是在芽孢形成过程中形成无活力包涵体或伴孢体的几种生物体之一。这个体是球形的，可能与孢子一样大或比孢子更大。它在细胞中的位置不是随机的；孢子是末端的，而体是近中心的，位于孢子和其余营养细胞染色质体之间。芽孢形成完成后，孢子和体都包含在芽孢外壁内。在经高锰酸盐固定的材料的超薄切片中追踪了细胞结构的发育顺序。当芽孢形成充分进展时，体开始从单晶生长，然后大概由于生长过程中的一些取向紊乱，它发展为多晶体，其晶格以不同角度取向。当体在大小上接近孢子时，形成一层薄片包膜，并且芽孢外壁发育，最终包围体和孢子。其他在显微镜下与围绕伴孢体的那些相似的薄片系统在芽孢外壁外的细胞质中自由发育。在这两个系统中，薄片的数量是可变的。福勒氏芽孢杆菌的芽孢衣由外层薄片层和内层未分辨的无定形层组成，在显微镜下已发现与蜡状芽孢杆菌的芽孢衣相同。在这两种生物体中，与其他薄片系统相比，芽孢衣的薄片层由规则数量的薄片组成。生理测试表明福勒氏芽孢杆菌是蜡状芽孢杆菌的一个变种。
