HARDY F M, BROWN A
J Bacteriol. 1961 Sep;82(3):449-57. doi: 10.1128/jb.82.3.449-457.1961.
Hardy, Frank M. (U. S. Army Chemical Corps, Fort Detrick, Frederick, Maryland), and Arthur Brown. Growth of Venezuelan equine encephalomyelitis virus in L cells. II. Growth in submerged culture. J. Bacteriol. 82:449-457. 1961.-The detailed kinetics of Venezuelan equine encephalomyelitis virus multiplication and release and the progressive cell death due to the virus infection were studied in a uniformly susceptible L cell population. The adsorption phase was complete in approximately 15 min with multiplicities of 1, 10, and 100 MICLD(50). An eclipse phase showing the disappearance of some of the infectious virus activity lasted between 3 and 4 hr. An exponential increase of cell-associated and free virus then occurred until peak titer was reached (16 to 18 hr). Relatively constant titers of cell-associated or free virus were obtained from the time peak titer was attained until the 52nd hour of incubation. At that time the cell-associated titer declined more rapidly than the free virus titer. These constant titers, however, masked a continued production and release of new infectious virus. By viable and nonviable cell counts, it was determined that cell death occurred relatively late in the phase of constant growth and release.
哈迪，弗兰克·M.（美国陆军化学兵团，马里兰州弗雷德里克市德特里克堡），以及亚瑟·布朗。委内瑞拉马脑炎病毒在L细胞中的生长。II. 悬浮培养中的生长。《细菌学杂志》82:449 - 457。1961年。——在一个对病毒均一敏感的L细胞群体中，研究了委内瑞拉马脑炎病毒增殖、释放的详细动力学过程以及病毒感染导致的细胞逐渐死亡。在感染复数为1、10和100个最小感染量（50）时，吸附阶段大约在15分钟内完成。一个显示部分感染性病毒活性消失的隐蔽期持续3至4小时。随后细胞相关病毒和游离病毒呈指数增长，直至达到峰值滴度（16至18小时）。从达到峰值滴度之时起直到培养52小时，细胞相关病毒或游离病毒的滴度相对恒定。然而，在这个恒定滴度阶段，新的感染性病毒仍在持续产生和释放。通过活细胞和死细胞计数确定，细胞死亡发生在恒定生长和释放阶段相对较晚的时候。
