委内瑞拉马脑炎病毒的体外复制。II. 悬浮培养物中病毒对选定营养添加剂的生长反应。
Replication of Venezuelan equine encephalomyelitis virus in vitro. II. Viral growth response to selected nutritional additives in suspension cultures.
作者信息
Hearn H J, Tribble H R, Bowersox O C, Nagle S C
出版信息
Appl Microbiol. 1971 Feb;21(2):342-5.
An attempt was made to identify nutritional additives that influence the replication of Venezuelan equine encephalomyelitis virus in suspension cultures grown in a defined serum-free medium. Proline, serine, and choline enhanced titers of the virulent PES strain; the progeny population, however, possessed a virulence character that was somewhat different from that of the PES inocula. These nutritional supplements did not appreciably influence the titers of the attenuated 9t and 20t viral strains. When both the PES and 20t strains were employed as a mixed inoculum in culture, the presence of the latter strain appeared to interfere with the growth of the PES strain and reduced its response to the medium supplements.
人们试图鉴定出在限定的无血清培养基中生长的悬浮培养物里，能够影响委内瑞拉马脑炎病毒复制的营养添加剂。脯氨酸、丝氨酸和胆碱可提高强毒株PES的滴度；然而，子代群体具有的毒力特征与PES接种物的毒力特征有所不同。这些营养补充剂对减毒株9t和20t的病毒滴度没有明显影响。当PES和20t毒株在培养中作为混合接种物使用时，后一种毒株的存在似乎会干扰PES毒株的生长，并降低其对培养基补充剂的反应。
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