Andersen P S, Smith J M, Mygind B
Enzyme Division, University of Copenhagen, Denmark.
Eur J Biochem. 1992 Feb 15;204(1):51-6. doi: 10.1111/j.1432-1033.1992.tb16604.x.
The upp gene coding for uracil phosphoribosyltransferase was subcloned on a 5-kb EcoRI restriction fragment along with the purMN operon. By a combination of complementation, deletion and minicell analyses, the upp gene was located adjacent to and divergently transcribed from the purMN operon. All three gene products could be identified in minicell extracts. The cloned upp gene shows an elevated expression upon uracil starvation. The nucleotide sequence and transcription start of the gene were determined. The sequence yields an open reading frame of 624 nucleotides encoding a protein of 22.5 kDa which is in agreement with the previously determined subunit Mr of the purified enzyme. A putative 5-phosphoribosyl-alpha-1-diphosphate (PRPP) binding site has been identified which is similar to the PRPP binding site of the yeast uracil phosphoribosyltransferase.
编码尿嘧啶磷酸核糖基转移酶的upp基因与purMN操纵子一起亚克隆到一个5 kb的EcoRI限制片段上。通过互补、缺失和小细胞分析相结合的方法，确定upp基因位于purMN操纵子相邻且转录方向相反的位置。在小细胞提取物中可以鉴定出所有三种基因产物。克隆的upp基因在尿嘧啶饥饿时表达升高。测定了该基因的核苷酸序列和转录起始位点。该序列产生一个624个核苷酸的开放阅读框，编码一个22.5 kDa的蛋白质，这与先前测定的纯化酶的亚基分子量一致。已鉴定出一个假定的5-磷酸核糖-α-1-二磷酸（PRPP）结合位点，它与酵母尿嘧啶磷酸核糖基转移酶的PRPP结合位点相似。
