Juranka P, Zhang F, Kulpa J, Endicott J, Blight M, Holland I B, Ling V
Ontario Cancer Institute, University of Toronto, Canada.
J Biol Chem. 1992 Feb 25;267(6):3764-70.
The prokaryotic hlyB gene product is a member of a superfamily of ATP-binding transport proteins that include the eukaryotic multidrug-resistance P-glycoprotein, the yeast STE6, and the cystic fibrosis CFTR gene products (Juranka, P. F., Zastawny, R. L., and Ling, V. (1989) FASEB J. 3, 2583-2592). Previous genetic studies have indicated that HlyB is involved in the transport of the 107-kDa HlyA protein from Escherichia coli; however, the HlyB protein has not been purified for biochemical studies due to its low abundance. In this study, we have engineered a monoclonal antibody epitope into the C-terminal end of HlyB that did not destroy its function. This has allowed us to use immunological methods to identify and localize various molecular forms of the HlyB protein present in vivo.
原核生物hlyB基因产物是ATP结合转运蛋白超家族的成员，该超家族包括真核生物多药耐药P-糖蛋白、酵母STE6以及囊性纤维化CFTR基因产物（尤兰卡，P.F.，扎斯塔尼，R.L.，和凌，V.（1989年）《美国实验生物学会联合会杂志》3，2583 - 2592）。先前的遗传学研究表明，HlyB参与大肠杆菌107 kDa HlyA蛋白的转运；然而，由于其丰度较低，HlyB蛋白尚未被纯化用于生化研究。在本研究中，我们在HlyB的C末端设计了一个单克隆抗体表位，该表位不会破坏其功能。这使我们能够使用免疫学方法来鉴定和定位体内存在的各种分子形式的HlyB蛋白。
