产黑素拟杆菌的一个解糖菌株对L-天冬氨酸的发酵作用
Fermentation of L-aspartate by a saccharolytic strain of Bacteroides melaninogenicus.
作者信息
Wong J C, Dyer J K, Tribble J L
出版信息
Appl Environ Microbiol. 1977 Jan;33(1):69-73. doi: 10.1128/aem.33.1.69-73.1977.
Resting cells of Bacteroides melaninogenicus fermented L-[14C]aspartate as a single substrate. The 14C-labeled products included succinate, acetate, CO2, oxaloacetate, formate, malate, glycine, alanine, and fumarate in the relative percentages 68, 15, 9.9, 2.7, 1.8, 1.0, 0.7, 0.5, and 0.06, respectively, based on the total counts per minute of the L-[14C]aspartate fermented. Ammonia was produced in high amounts, indicating that 96% of the L-aspartate fermented was deaminated. These data suggest that L-aspartate is mainly being reduced through a number of intermediate reactions involving enzymes of the tricarboxylic acid cycle to succinate. L-[14C]asparagine was also fermented by resting cells of B. melaninogenicus to form L-aspartate, which was subsequently, but less actively, fermented.
产黑素拟杆菌的静息细胞以L-[¹⁴C]天冬氨酸作为单一底物进行发酵。¹⁴C标记的产物包括琥珀酸、乙酸、二氧化碳、草酰乙酸、甲酸、苹果酸、甘氨酸、丙氨酸和富马酸，其相对百分比分别为68%、15%、9.9%、2.7%、1.8%、1.0%、0.7%、0.5%和0.06%，基于发酵的L-[¹⁴C]天冬氨酸的每分钟总计数。大量产生了氨，表明发酵的L-天冬氨酸中有96%被脱氨。这些数据表明，L-天冬氨酸主要通过一系列涉及三羧酸循环酶的中间反应被还原为琥珀酸。L-[¹⁴C]天冬酰胺也被产黑素拟杆菌的静息细胞发酵形成L-天冬氨酸，随后L-天冬氨酸被发酵，但活性较低。
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