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Expression of Escherichia coli homoserine kinase in mouse 3T3 cells.

作者信息

Rees W D, Hay S M, Flint H J

机构信息

Rowett Research Institute, Scotland, U.K.

出版信息

Biochem J. 1992 Feb 1;281 ( Pt 3)(Pt 3):865-70. doi: 10.1042/bj2810865.

DOI:10.1042/bj2810865
PMID:1371387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1130769/
Abstract

The Escherichia coli gene for homoserine kinase (thrB) has been cloned into a simian-virus-40-based eukaryotic expression vector which also includes a neomycin-resistance gene. Mouse 3T3 cells transfected with this plasmid were selected for resistance and screened for homoserine kinase activity. It has thus been possible to isolate clones which are capable of accumulating homoserine O-phosphate when supplied with homoserine. In broken-cell preparations the kinetic constants for the production of homoserine O-phosphate were similar to those of the wild-type E. coli enzyme. These experiments demonstrate that E. coli homoserine kinase can be expressed in an animal cell and that it can successfully phosphorylate L-homoserine in the intact cell utilizing endogenous ATP.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/1130769/0b3f32521183/biochemj00142-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/1130769/6238874705bd/biochemj00142-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/1130769/0b3f32521183/biochemj00142-0281-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/1130769/6238874705bd/biochemj00142-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a26/1130769/0b3f32521183/biochemj00142-0281-a.jpg

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引用本文的文献

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Biochem J. 1994 May 1;299 ( Pt 3)(Pt 3):637-44. doi: 10.1042/bj2990637.
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LYSINE BIOSYNTHESIS IN YEAST: A NEW METABOLITE OF ALPHA-AMINOADIPIC ACID.酵母中的赖氨酸生物合成：α-氨基己二酸的一种新代谢物
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Proc Natl Acad Sci U S A. 1982 Jan;79(2):257-61. doi: 10.1073/pnas.79.2.257.
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Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.特定pBR322 DNA序列对猴细胞中SV40复制的抑制作用。
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