大肠杆菌thrB基因及其两个相邻区域的核苷酸序列；thrAB和thrBC连接点。
Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions.
作者信息
Cossart P, Katinka M, Yaniv M
出版信息
Nucleic Acids Res. 1981 Jan 24;9(2):339-47. doi: 10.1093/nar/9.2.339.
Abstract
We have sequenced a DNA fragment containing the Escherichia coli thrA-thrB junction, the complete thrB gene and the thrB-thrC junction. The intergenic sequence thrA and thrB is only one base pair. The coding region for homoserine kinase is 927 base pairs long. It is followed by 114 base pair segment in an open reading frame predicting that thrC begins just after non-sense codon of thrB. The presence at the end of thrA and of thrB of sequences that can pair with the 3' end of the 16 S ribosomal RNA suggests that reinitiation of translation occurs at the end of the two genes. The deduced aminoacid sequence for homoserine kinase shows no striking homology with aspartokinase I homoserine dehydrogenase I.
摘要
我们对一段DNA片段进行了测序，该片段包含大肠杆菌的thrA - thrB连接区、完整的thrB基因以及thrB - thrC连接区。thrA和thrB之间的基因间序列仅有一个碱基对。高丝氨酸激酶的编码区长度为927个碱基对。其后是一个114个碱基对的片段，处于一个开放阅读框中，预示着thrC恰好在thrB的无义密码子之后开始。thrA和thrB末端存在可与16 S核糖体RNA的3'末端配对的序列，这表明在这两个基因的末端发生了翻译重新起始。推导得出的高丝氨酸激酶氨基酸序列与天冬氨酸激酶I - 高丝氨酸脱氢酶I没有明显的同源性。
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