Hitchcock R J, Pemble M J, Bishop A E, Spitz L, Polak J M
Department of Paediatric Surgery, Royal Postgraduate Medical School, London, England.
Gastroenterology. 1992 Mar;102(3):840-8. doi: 10.1016/0016-5085(92)90167-w.
The innervation and neuropeptide expression of fetal and infant human esophagus were studied. Esophageal samples (n = 30) from 8 weeks' gestation to 28 months of age were immunostained using antisera to general and specific neuronal antigens, and the results were quantified using computer-assisted image analysis. Nerve protein (protein gene peptide 9.5 and synaptophysin) and glial cell protein (S100) immunoreactivities were present by 8 weeks' gestation in primitive cell bodies and fibers in the outer layers of the esophagus. Immunoreactivity for peptides was first detected in fibers at 11 weeks' gestation in myenteric plexus and at 13 weeks' gestation in muscle. Peptide-immunoreactive cell bodies were not seen until 13-15 weeks. A pattern of immunoreactivity for neuropeptides comparable with that seen in mature neonates and infants was present by 22 weeks of gestational age. The percentage area of protein gene peptide 9.5-immunoreactive and vasoactive intestinal peptide-immunoreactive nerve fibers increased from low levels to 3.68% and 0.27%, respectively, at 13 weeks and peaked at 18 weeks (10.50% and 4.74%). These findings provide a foundation for future research into the contribution of neuropeptides to pediatric esophageal dysmotility.
对胎儿及婴儿期人类食管的神经支配和神经肽表达进行了研究。使用针对一般和特定神经元抗原的抗血清，对妊娠8周龄至28月龄的食管样本（n = 30）进行免疫染色，并采用计算机辅助图像分析对结果进行定量。妊娠8周时，在食管外层的原始细胞体和纤维中可检测到神经蛋白（蛋白基因肽9.5和突触素）及胶质细胞蛋白（S100）的免疫反应性。肽类的免疫反应性最早在妊娠11周时于肌间神经丛的纤维中检测到，在妊娠13周时于肌肉中检测到。直到妊娠13 - 15周才可见到肽免疫反应性细胞体。到妊娠22周时，神经肽的免疫反应性模式与成熟新生儿和婴儿中所见的模式相当。蛋白基因肽9.5免疫反应性和血管活性肠肽免疫反应性神经纤维的面积百分比从低水平分别在13周时增加到3.68%和0.27%，并在18周时达到峰值（10.50%和4.74%）。这些发现为未来研究神经肽对小儿食管动力障碍的作用奠定了基础。
