凝集素与小鼠T淋巴细胞的结合模式。
Pattern of lectin binding to murine T lymphocytes.
作者信息
Sowalsky R A, Fox B S
机构信息
Department of Medicine, School of Medicine, University of Maryland, Baltimore.
出版信息
Immunology. 1992 Jan;75(1):92-8.
Lectins can be used to detect changes in cell-surface glycosylation. In this paper we examine the lectin-binding characteristics of murine T cells as measured by flow cytometry. A large panel of labelled lectins was used to stain naive, activated and resting murine T cells. Some lectins did not detectably bind any T cells, some bound to all T cells and some bound to only a subset of splenic T cells. Three lectins which preferentially bind to previously activated T cells were identified: Bandeiraea simplicifolia BS-I, Bauhinia purpurea and Lycopersicon esculentum. In addition, two lectins were found to bind preferentially to activated T cells: Glycine max and Triticum vulgaris. Finally, no differences were found in the ability of a large panel of lectins to bind to Th1 and Th2 clones. Lectin binding may be a powerful tool for distinguishing naive, activated and memory T cells.
凝集素可用于检测细胞表面糖基化的变化。在本文中，我们通过流式细胞术检测了小鼠T细胞的凝集素结合特性。使用一大组标记的凝集素对未激活、激活和静止的小鼠T细胞进行染色。一些凝集素未检测到与任何T细胞结合，一些与所有T细胞结合，还有一些仅与脾脏T细胞的一个亚群结合。鉴定出三种优先结合先前激活的T细胞的凝集素：单叶豆（Bandeiraea simplicifolia）BS-I、紫羊蹄甲（Bauhinia purpurea）和番茄（Lycopersicon esculentum）。此外，发现两种凝集素优先结合激活的T细胞：大豆（Glycine max）和小麦（Triticum vulgaris）。最后，一大组凝集素与Th1和Th2克隆结合的能力未发现差异。凝集素结合可能是区分未激活、激活和记忆T细胞的有力工具。
Zotero 插件