Whyte A, Garratt L, James P S, Binns R M
Department of Immunology, AFRC Babraham Institute, Cambridge, UK.
Histochem J. 1993 Oct;25(10):726-34. doi: 10.1007/BF00211768.
The distribution of saccharides in pig lymph nodes, particularly on high-endothelial venule (HEV) endothelium and on lymphocytes in these vessels, was studied by examining the binding of fluorescent conjugates of 18 different lectins. Eight of the lectins, particularly with glycan specificity restricted to mannose and polyacetyllactosamine determinants, were found to bind with a high affinity to these structures. Competitive inhibition experiments revealed that polylactosamine-containing glycans were present on endothelia and lymphocytes using lectins from Lycopersicon esculentum and Solanum tuberosum, the latter lectin reacting with lymphocytes only when apparently adherent to the luminal endothelium. The The absence on pig endothelium of the Ulex europaeus binding, shown by human endothelia due to the presence of certain fucose epitopes, was confirmed. Pig lymph-node endothelium, however, bound the fucose-specific lectin of Tetragonolobus purpureas, indicating the presence of fucose on pig endothelia in a different conformation to that seen on human endothelia. The results suggested that pig lymph-node HEV endothelium expressed a core fucosylated tri- or tetra-antennary complex glycan with polylactosamine extensions and expressing an Ley determinant.
通过检测18种不同凝集素的荧光缀合物的结合情况，研究了糖类在猪淋巴结中的分布，特别是在高内皮微静脉（HEV）内皮细胞和这些血管中的淋巴细胞上的分布。发现其中8种凝集素，特别是那些聚糖特异性仅限于甘露糖和聚乙酰乳糖胺决定簇的凝集素，能与这些结构高亲和力结合。竞争性抑制实验表明，使用番茄凝集素和马铃薯凝集素时，内皮细胞和淋巴细胞上存在含聚乳糖胺的聚糖，后者凝集素仅在明显粘附于管腔内皮时才与淋巴细胞发生反应。证实了猪内皮细胞不存在欧洲荆豆凝集素结合，而人内皮细胞因存在某些岩藻糖表位而有这种结合。然而，猪淋巴结内皮细胞结合了紫花四棱豆的岩藻糖特异性凝集素，表明猪内皮细胞上存在岩藻糖，其构象与人内皮细胞上的不同。结果表明，猪淋巴结HEV内皮细胞表达一种具有聚乳糖胺延伸的核心岩藻糖基化三或四天线复杂聚糖，并表达Ley决定簇。
