Baylor N W, Fu T, Yan Y D, Ruscetti F W
Biological Carcinogenesis and Development Program, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland.
J Infect Dis. 1992 Mar;165(3):433-7. doi: 10.1093/infdis/165.3.433.
The ability of baicalin (7-glucuronic acid, 5,6-dihydroxyflavone), a flavonoid compound purified from the Chinese medicinal herb, Scutellaria baicalensis georgi, to inhibit human T cell leukemia virus type I (HTLV-I) was examined. Baicalin produced concentration-dependent inhibition of HTLV-I replication in productively infected T and B cells. Moreover, baicalin treatment selectively reduced the detectable levels of HTLV-I p19 gag protein in infected cells by greater than 70% at concentrations that produced insignificant effects on total cellular protein and DNA synthesis with no loss in cell viability. Resistance to HTLV-I infection and virus-mediated transformation was noted in uninfected peripheral blood lymphocytes pretreated with baicalin before cocultivation with lethally irradiated chronically infected cells. Baicalin inhibited reverse transcriptase activity in HTLV-I-infected cells as well as the activity of purified reverse transcriptase from Moloney murine leukemia virus and Rous-associated virus type 2. These results suggest that baicalin may be a potential therapeutic agent against HTLV-I-associated T cell diseases.
对从中药材黄芩中提纯的黄酮类化合物黄芩苷（7-葡萄糖醛酸，5,6-二羟基黄酮）抑制人I型T细胞白血病病毒（HTLV-I）的能力进行了研究。黄芩苷对受HTLV-I感染并处于活跃复制状态的T细胞和B细胞的复制产生浓度依赖性抑制作用。此外，在对总细胞蛋白和DNA合成无显著影响且不降低细胞活力的浓度下，黄芩苷处理能使受感染细胞中HTLV-I p19 gag蛋白的可检测水平选择性降低70%以上。在用黄芩苷预处理的未感染外周血淋巴细胞与经致死性照射的慢性感染细胞共培养后，观察到其对HTLV-I感染和病毒介导的转化具有抗性。黄芩苷可抑制HTLV-I感染细胞中的逆转录酶活性以及来自莫洛尼氏鼠白血病病毒和2型劳斯相关病毒的纯化逆转录酶的活性。这些结果表明，黄芩苷可能是一种针对HTLV-I相关T细胞疾病的潜在治疗药物。
