Tanaka Y, Masuda M, Yoshida A, Shida H, Nyunoya H, Shimotohno K, Tozawa H
Department of Immunology, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
AIDS Res Hum Retroviruses. 1992 Feb;8(2):227-35. doi: 10.1089/aid.1992.8.227.
In order to study an antigenic structure of the trans-activator protein encoded by human T-cell leukemia virus type-I (HTLV-I), tax1 antigen, we generated and characterized a panel of rat anti-tax1 monoclonal antibodies (MAbs) designated WATM-1, WATM-2, WATM-3, and WATM-4. These MAbs were derived from WKA rats immunized with HTLV-I-transformed (HTLV-I+) syngeneic T cells. Immunoblot assays showed that: (1) All the MAbs reacted with the tax1 antigen in HTLV-I+ cell lines and a recombinant tax1 antigen, PX141 (containing entire tax1 polypeptide); (2) WATM-3 and WATM-4, but not WATM-1 or WATM-2, reacted with a truncated tax1 antigen, XD59 (tax1 amino acids 180-338); (3) None of them reacted with another truncated tax1 antigen, XD128 (tax1 amino acids 1-47 and 286-353); and (4) each of the four MAbs had different reactivity with tax1-related antigens in the range 38-41 kDa expressed in simian cell lines infected with various HTLV-I-related simian retroviruses (STLV-I). None of the MAbs reacted with HTLV-II tax antigen. Human sera containing anti-tax1 antibodies interfered specifically with the antigen-specific binding of all the MAbs. These results suggest that the present rat MAbs are directed against various epitopes on the tax1 antigen. An antigenic structure of the tax1 antigen deduced from reactivity of a panel of anti-tax1 MAbs including the present rat MAbs is discussed.
为了研究人类I型T细胞白血病病毒（HTLV-I）编码的反式激活蛋白tax1抗原的抗原结构，我们制备并鉴定了一组大鼠抗tax1单克隆抗体（MAb），命名为WATM-1、WATM-2、WATM-3和WATM-4。这些单克隆抗体源自用HTLV-I转化的（HTLV-I+）同基因T细胞免疫的WKA大鼠。免疫印迹分析表明：（1）所有单克隆抗体都与HTLV-I+细胞系中的tax1抗原和重组tax1抗原PX141（包含完整的tax1多肽）发生反应；（2）WATM-3和WATM-4与截短的tax1抗原XD59（tax1氨基酸180 - 338）发生反应，而WATM-1或WATM-2则不反应；（3）它们都不与另一种截短的tax1抗原XD128（tax1氨基酸1 - 47和286 - 353）发生反应；（4）这四种单克隆抗体中的每一种与感染各种HTLV-I相关猿猴逆转录病毒（STLV-I）的猿猴细胞系中表达的38 - 41 kDa范围内的tax1相关抗原具有不同的反应性。没有一种单克隆抗体与HTLV-II tax抗原发生反应。含有抗tax1抗体的人血清特异性干扰所有单克隆抗体的抗原特异性结合。这些结果表明，目前的大鼠单克隆抗体针对tax1抗原上的各种表位。本文讨论了从包括目前大鼠单克隆抗体在内的一组抗tax1单克隆抗体的反应性推导得出的tax1抗原的抗原结构。
