Ishikawa R, Nishikori K, Furukawa Y, Hayashi K, Furukawa S
Department of Molecular Biology, Gifu Pharmaceutical University, Japan.
Neurosci Lett. 1992 Jan 20;135(1):113-6. doi: 10.1016/0304-3940(92)90148-z.
Acidic fibroblast growth factor (aFGF) level in sciatic nerve after lesioning was measured by enzyme immunoassay to determine if aFGF functions as a neurotrophic factor like nerve growth factor (NGF). Whereas the NGF level increased in distal segments, the aFGF level there decreased after transection or crushing and recovered to the original level by 10 weeks after crushing. The amount of aFGF mRNA in the sciatic nerve was extremely low to supply the high level of protein found in the sciatic nerve. Sympathetic ganglia, dorsal root ganglia, and spinal cord, which contain neuronal cell bodies extending their axons into the sciatic nerve, showed a greater or similar level of aFGF as sciatic nerve. These results imply that aFGF is synthesized in neuronal cell bodies and distributed anterogradely into their axons. Difference of injury-induced changes in levels between aFGF and NGF suggests distinct mechanisms of the effects elicited from these factors on regeneration of the sciatic nerve.
通过酶免疫测定法测量损伤后坐骨神经中的酸性成纤维细胞生长因子（aFGF）水平，以确定aFGF是否像神经生长因子（NGF）一样发挥神经营养因子的作用。虽然在远侧段NGF水平升高，但在横断或挤压后，那里的aFGF水平下降，并在挤压后10周恢复到原始水平。坐骨神经中aFGF mRNA的量极低，无法提供坐骨神经中发现的高水平蛋白质。交感神经节、背根神经节和脊髓含有将轴突延伸到坐骨神经中的神经元细胞体，其aFGF水平与坐骨神经相当或更高。这些结果表明，aFGF在神经元细胞体中合成，并沿轴突顺行分布。aFGF和NGF损伤诱导水平变化的差异表明这些因子对坐骨神经再生产生影响的机制不同。
