Chen Bing, Hu Rong, Min Qing, Li Yankun, Parkinson David B, Dun Xin-Peng
Department of Neurology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Huai'an, China.
School of Traditional Chinese Medicine, Southern Medical School, Guangzhou, China.
Front Cell Neurosci. 2020 Aug 4;14:237. doi: 10.3389/fncel.2020.00237. eCollection 2020.
The fibroblast growth factor (FGF) family polypeptides play key roles in promoting tissue regeneration and repair. FGF5 is strongly up-regulated in Schwann cells of the peripheral nervous system following injury; however, a role for FGF5 in peripheral nerve regeneration has not been shown up to now. In this report, we examined the expression of FGF5 and its receptors FGFR1-4 in Schwann cells of the mouse sciatic nerve following injury, and then measured the effects of FGF5 treatment upon cultured primary rat Schwann cells. By microarray and mRNA sequencing data analysis, RT-PCR, qPCR, western blotting and immunostaining, we show that FGF5 is highly up-regulated in Schwann cells of the mouse distal sciatic nerve following injury, and FGFR1 and FGFR2 are highly expressed in Schwann cells of the peripheral nerve both before and following injury. Using cultured primary rat Schwann cells, we show that FGF5 inhibits ERK1/2 MAP kinase activity but promotes rapid Schwann cell migration and adhesion the upregulation of N-cadherin. Thus, FGF5 is an autocrine regulator of Schwann cells to regulate Schwann cell migration and adhesion.
成纤维细胞生长因子（FGF）家族多肽在促进组织再生和修复中起关键作用。FGF5在外周神经系统损伤后的施万细胞中强烈上调；然而，迄今为止FGF5在外周神经再生中的作用尚未得到证实。在本报告中，我们检测了损伤后小鼠坐骨神经施万细胞中FGF5及其受体FGFR1 - 4的表达，然后测定了FGF5处理对原代培养大鼠施万细胞的影响。通过微阵列和mRNA测序数据分析、RT - PCR、qPCR、蛋白质免疫印迹和免疫染色，我们发现损伤后小鼠坐骨神经远端的施万细胞中FGF5高度上调，且FGFR1和FGFR2在损伤前后的外周神经施万细胞中均高表达。利用原代培养的大鼠施万细胞，我们发现FGF5抑制ERK1/2丝裂原活化蛋白激酶活性，但促进施万细胞快速迁移和黏附——N - 钙黏蛋白上调。因此，FGF5是施万细胞的自分泌调节因子，可调节施万细胞的迁移和黏附。
