Groves R W, Ross E, Barker J N, Ross J S, Camp R D, MacDonald D M
St. John's Institute of Dermatology, UMDS, London, U.K.
J Invest Dermatol. 1992 Mar;98(3):384-7. doi: 10.1111/1523-1747.ep12499816.
We have examined the expression of three endothelial adhesion molecules (intercellular adhesion molecule-1 [ICAM-1], endothelial leukocyte adhesion molecule-1 [ELAM-1], and vascular cell adhesion molecule-1 [VCAM-1]) in normal human skin following intradermal injection of stratum corneum-derived interleukin-1 alpha (SCIL-1 alpha). In control skin, constitutive expression of ICAM-1 was found on endothelial cells and at low levels on dermal dendritic cells but not on keratinocytes. ELAM-1 and VCAM-1 were present in low levels on endothelium and perivascular dendritic cells, respectively. SCIL-1 alpha injection produced marked endothelial ELAM-1 upregulation. Double staining with neutrophil elastase demonstrated that many ELAM-1-positive vessels contained marginating neutrophils and that interstitial neutrophils were clustered around ELAM-1-positive vessels. An increase in dermal dendritic cell ICAM-1 expression occurred and in two of three biopsies there was keratinocyte expression of ICAM-1 in the SCIL-1 alpha-injected tissue. Also, there was upregulation of VCAM-1 on vascular endothelium and an increase in the dermal dendritic cell expression of this molecule. These results give in vivo confirmation that SCIL-1 alpha modulated endothelial and dermal dendritic cell adhesion molecule expression, and show that endothelial VCAM-1 is regulated in vivo by SCIL-1 alpha, thus providing a regulatable ICAM-1-independent means of mononuclear cell recruitment.
我们检测了在皮内注射角质层来源的白细胞介素-1α（SCIL-1α）后，正常人皮肤中三种内皮细胞黏附分子（细胞间黏附分子-1 [ICAM-1]、内皮白细胞黏附分子-1 [ELAM-1]和血管细胞黏附分子-1 [VCAM-1]）的表达情况。在对照皮肤中，ICAM-1的组成性表达在内皮细胞上被发现，在真皮树突状细胞上表达水平较低，但在角质形成细胞上未发现表达。ELAM-1和VCAM-1分别在内皮细胞和血管周围树突状细胞上低水平存在。注射SCIL-1α后导致内皮细胞ELAM-1显著上调。用中性粒细胞弹性蛋白酶进行双重染色显示，许多ELAM-1阳性血管中有边缘化的中性粒细胞，并且间质中性粒细胞聚集在ELAM-1阳性血管周围。真皮树突状细胞ICAM-1表达增加，并且在三份活检样本中的两份中，注射SCIL-1α的组织中有角质形成细胞表达ICAM-1。此外，血管内皮细胞上的VCAM-1上调，并且该分子在真皮树突状细胞中的表达增加。这些结果在体内证实了SCIL-1α调节内皮细胞和真皮树突状细胞黏附分子的表达，并表明内皮细胞VCAM-1在体内受SCIL-1α调节，从而提供了一种可调节的、不依赖ICAM-1的单核细胞募集方式。
