Arlett C F, Harcourt S A, Cole J, Green M H, Anstey A V
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, Great Britain.
Mutat Res. 1992 Mar;273(2):127-35. doi: 10.1016/0921-8777(92)90074-d.
Unstimulated T-lymphocytes from normal donors are significantly more sensitive to the lethal effects of UV-C than either stimulated T-lymphocytes or fibroblasts as judged by colony-forming ability. Data from other studies suggest that excision repair is more effective in stimulated than unstimulated T-lymphocytes leading to the prediction that these differences in survival should be minimal in cells established from excision defective donors. The prediction was met with XP6BR, a donor of unknown complementation group. For 3 XP's from complementation group D, however, enhanced survival in stimulated T-cells was observed. With cells from an excision-defective TTD who was included in complementation group D of XP both fibroblasts and unstimulated T-lymphocytes were hypersensitive. For a second excision defective TTD patient who was excluded from complementation group D, the unstimulated T-lymphocytes were more resistant than those of normal donors although the fibroblasts were hypersensitive. These results suggest that the in vitro response of stimulated T-lymphocytes or fibroblasts may not reflect the in vivo response of cells, as measured by the response of unstimulated T-lymphocytes.
根据集落形成能力判断，来自正常供体的未受刺激的T淋巴细胞对UV-C的致死效应比受刺激的T淋巴细胞或成纤维细胞更敏感。其他研究的数据表明，切除修复在受刺激的T淋巴细胞中比在未受刺激的T淋巴细胞中更有效，这导致预测这些生存差异在来自切除缺陷供体的细胞中应最小。这一预测在XP6BR（一个互补组未知的供体）中得到了验证。然而，对于来自互补组D的3名XP患者，观察到受刺激的T细胞存活率提高。对于一名被纳入XP互补组D的切除缺陷型TTD患者的细胞，成纤维细胞和未受刺激的T淋巴细胞都高度敏感。对于另一名被排除在互补组D之外的切除缺陷型TTD患者，未受刺激的T淋巴细胞比正常供体的更具抗性，尽管成纤维细胞高度敏感。这些结果表明，受刺激的T淋巴细胞或成纤维细胞的体外反应可能无法反映未受刺激的T淋巴细胞反应所衡量的细胞体内反应。
