Green M H, Lowe J E, Harcourt S A, Akinluyi P, Rowe T, Cole J, Anstey A V, Arlett C F
MRC Cell Mutation Unit, Sussex University, Falmer, Brighton, Great Britain.
Mutat Res. 1992 Mar;273(2):137-44. doi: 10.1016/0921-8777(92)90075-e.
We have studied incision-break formation in unstimulated and stimulated populations of human T-lymphocytes using the comet (single-cell microgel electrophoresis) assay. The frequency of strand breaks 1 h after UV-irradiation appears to be far greater in unstimulated than in stimulated lymphocytes from normal donors and the excess of strand breaks was observed for a far longer time after irradiation. This result corroborates the greater sensitivity of UV-C irradiation observed in a colony-forming assay but suggests that the defect may relate to a defect in strand rejoining rather than a defect in incision. Few strand breaks were seen in either unstimulated or stimulated lymphocytes of four xeroderma pigmentosum donors, suggesting that the method may offer a rapid diagnostic assay for XP.
我们使用彗星试验（单细胞微凝胶电泳）研究了未刺激和刺激状态下的人T淋巴细胞群体中的切口断裂形成情况。紫外线照射1小时后，来自正常供体的未刺激淋巴细胞中的链断裂频率似乎远比刺激淋巴细胞中的高，并且在照射后很长一段时间内都观察到链断裂过量。这一结果证实了在集落形成试验中观察到的UV-C照射的更高敏感性，但表明缺陷可能与链重新连接缺陷有关，而非切口缺陷。在四名着色性干皮病供体的未刺激或刺激淋巴细胞中均未观察到多少链断裂，这表明该方法可能为着色性干皮病提供一种快速诊断检测方法。
