Wallny H J, Rötzschke O, Falk K, Hämmerling G, Rammensee H G
Max-Planck-Institut für Biologie, Abteilung Immunogenetik, Tübingen, FRG.
Eur J Immunol. 1992 Mar;22(3):655-9. doi: 10.1002/eji.1830220307.
DBA/2-derived mouse tumor cells were transfected with the H-2 Kb gene. Naturally processed minor histocompatibility (H) peptides were extracted from both transfected and non-transfected cells by acid elution, and were separated by high-performance liquid chromatography. Kb-restricted minor H epitopes corresponding to H-4b and mapki, both encoded by non-major histocompatibility complex genes of DBA/2, were readily detected by the respective cytotoxic T lymphocyte in peptides extracted from Kb-transfected, but not from non-transfected or Db-transfected cells. Titration experiments indicated at least 3000-fold less copies of correctly processed Kb-restricted epitopes in cells without Kb as compared to cells with Kb. Since we estimate the copy number of Kb-restricted H-4b epitopes in Kb-expressing transfectants to be less than 1000 per cell, the pool size of H-4b epitopes correctly processed in the absence of Kb should be less than 1/3 copy per cell.
将源自DBA/2的小鼠肿瘤细胞用H-2 Kb基因进行转染。通过酸洗脱从转染和未转染的细胞中提取天然加工的次要组织相容性（H）肽，并通过高效液相色谱进行分离。分别由DBA/2的非主要组织相容性复合体基因编码的与H-4b和mapki相对应的Kb限制性次要H表位，在从Kb转染细胞而非未转染或Db转染细胞中提取的肽中，很容易被各自的细胞毒性T淋巴细胞检测到。滴定实验表明，与具有Kb的细胞相比，没有Kb的细胞中正确加工的Kb限制性表位的拷贝数至少少3000倍。由于我们估计在表达Kb的转染子中Kb限制性H-4b表位的拷贝数小于每个细胞1000个，因此在没有Kb的情况下正确加工的H-4b表位的库大小应小于每个细胞1/3个拷贝。
