Falk K, Rötzschke O, Faath S, Goth S, Graef I, Shastri N, Rammensee H G
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.
Cell Immunol. 1993 Sep;150(2):447-52. doi: 10.1006/cimm.1993.1212.
HeLa cells, derived from a human cervix carcinoma line, were transfected with a mouse MHC class I gene, H-2Kb, and chicken ovalbumin. H-2Kb-restricted cytotoxic mouse T cells specific for ovalbumin recognized the double-transfected human cells with similar efficiency as ovalbumin-transfected EL4 mouse thymoma cells (H-2b). The naturally processed ovalbumin T cell epitope was eluted from H-2Kb molecules from double-transfected HeLa cells and was biochemically compared to a synthetic peptide, SIINFEKL, known to be the natural Kb ligand of ovalbumin-transfected H-2b mouse cells. The results indicate that the ovalbumin-derived Kb-ligand of double-transfected HeLa cells is also SIINFEKL. Thus, both human cervix carcinoma cells and mouse thymoma cells expressing Kb and ovalbumin process the same octapeptide. Together with previous data, derived by comparing Kb ligands of unknown sequences from both human and mouse cells expressing Kb, it can be concluded that both mouse and human cells are capable of processing the same ligands for mouse MHC class I molecules. Hence, the general specificity of the peptide-generating mechanism for class I ligands is apparently conserved between evolutionary distant species.
源自人宫颈癌系的HeLa细胞用小鼠MHC I类基因H-2Kb和鸡卵清蛋白进行转染。对卵清蛋白特异的H-2Kb限制性细胞毒性小鼠T细胞识别双转染的人细胞，其效率与卵清蛋白转染的EL4小鼠胸腺瘤细胞（H-2b）相似。天然加工的卵清蛋白T细胞表位从双转染HeLa细胞的H-2Kb分子上洗脱下来，并与已知为卵清蛋白转染的H-2b小鼠细胞的天然Kb配体的合成肽SIINFEKL进行生化比较。结果表明，双转染HeLa细胞的源自卵清蛋白的Kb配体也是SIINFEKL。因此，表达Kb和卵清蛋白的人宫颈癌细胞和小鼠胸腺瘤细胞都加工相同的八肽。结合之前通过比较表达Kb的人和小鼠细胞中未知序列的Kb配体得出的数据，可以得出结论，小鼠和人细胞都能够加工相同的小鼠MHC I类分子配体。因此，I类配体的肽生成机制的一般特异性在进化上距离遥远的物种之间显然是保守的。
