Falk K, Rötzschke O, Rammensee H G
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, FRG.
Eur J Immunol. 1992 May;22(5):1323-6. doi: 10.1002/eji.1830220530.
Alloreactive mouse cytotoxic T lymphocytes (CTL) specific for particular peptides presented by H-2Kb molecules on mouse cells were found to recognize human cells transfected with Kb. The CTL-recognized peptides (probably derived from conserved proteins) were extracted from Kb-expressing human or mouse cells, respectively, and compared biochemically by high resolution high performance liquid chromatography. The results strongly suggest identity of peptides processed by cells from both species and thus indicate that the specificity of the processing machinery used in the major histocompatibility complex (MHC) class I-restricted pathway, probably including enzymes, transport mechanisms, and chaperons, is highly conserved across species. The results are consistent with the notion that MHC molecules themselves have an instructive role in processing.
发现对小鼠细胞上由H-2Kb分子呈递的特定肽具有同种反应性的小鼠细胞毒性T淋巴细胞（CTL）能够识别转染了Kb的人细胞。分别从表达Kb的人细胞或小鼠细胞中提取CTL识别的肽（可能源自保守蛋白），并通过高分辨率高效液相色谱法进行生化比较。结果强烈表明两种物种的细胞处理的肽具有同一性，因此表明主要组织相容性复合体（MHC）I类限制途径中使用的处理机制的特异性，可能包括酶、转运机制和伴侣蛋白，在物种间高度保守。这些结果与MHC分子本身在处理过程中具有指导作用的观点一致。
