Dale D C, Lau S, Nash R, Boone T, Osborne W
Department of Medicine, University of Washington, Seattle 98195.
J Infect Dis. 1992 Apr;165(4):689-94. doi: 10.1093/infdis/165.4.689.
The biologic effects of endotoxin are attributed to the release of several cytokines, including interleukin-1, interleukin-6, tumor necrosis factor, and the colony-stimulating factors. To investigate the mechanism of endotoxin-induced neutrophilia in dogs, several cell lines known to proliferate selectively in response to recombinant human colony-stimulating factors were examined to determine their responses to recombinant canine granulocyte colony-stimulating factor (rcG-CSF) or recombinant canine granulocyte-macrophage colony-stimulating factor (rcGM-CSF). The murine cell line NFS-60 was found to respond well to rcG-CSF and the human cell line TALL-101 to rcGM-CSF, and these responses were neutralized by antibodies to these recombinant proteins. These bioassays were then used to determine G-CSF and GM-CSF levels in dogs after intravenous endotoxin administration. G-CSF levels increased by 2 h, peaked at 4 h, and had not returned to normal by 24 h after endotoxin. In contrast, GM-CSF was not detectible before or after endotoxin administration.
内毒素的生物学效应归因于多种细胞因子的释放，包括白细胞介素-1、白细胞介素-6、肿瘤坏死因子和集落刺激因子。为了研究犬体内内毒素诱导中性粒细胞增多的机制，检测了几种已知对重组人集落刺激因子有选择性增殖反应的细胞系，以确定它们对重组犬粒细胞集落刺激因子（rcG-CSF）或重组犬粒细胞-巨噬细胞集落刺激因子（rcGM-CSF）的反应。发现鼠细胞系NFS-60对rcG-CSF反应良好，人细胞系TALL-101对rcGM-CSF反应良好，并且这些反应被针对这些重组蛋白的抗体所中和。然后使用这些生物测定法来测定犬静脉注射内毒素后G-CSF和GM-CSF的水平。内毒素注射后2小时G-CSF水平升高，4小时达到峰值，24小时后仍未恢复正常。相比之下，内毒素给药前后均未检测到GM-CSF。
