Meltzer M S, Oppenheim J J
J Immunol. 1977 Jan;118(1):77-82.
Adherent peritoneal cells (90 to 95% macrophages) from mice injected with Mycobacterium bovis, strain BCG, or certain noninfectious agents such as pyran copolymer or phytohemagglutinin showed increased chemotactic and tumoricidal capacity in vitro. These activated macrophages elaborated 2 to 5 times more lymphocyte-activating factor (LAF) in vitro than equal numbers of adherent cells from untreated mice. In contrast, adherent PC from mice treated with thioglycollate or mineral oil were not cytotoxic and did not produce more LAF than PC from untreated mice. Adherent PC from untreated nude mice, which have increased chemotactic and tumoricidal capacity in vitro, also exhibited enhanced LAF production compared to adherent PC from their normal littermates. Increased production of LAF was also evident with adherent PC and the macrophage-like tumor cell line P388D1 after incubation in vitro with bacterial endotoxins or with antigen-induced lymphokines. These data indicate that adherent PC can be activated either in vivo or in vitro to elaborate more LAF. Thus, activated macrophages are more effective than normal macrophages in amplification of the afferent limb of immune responses as well as in their effector functions.
给小鼠注射卡介苗（牛分枝杆菌菌株）或某些非感染性物质（如吡喃共聚物或植物血凝素）后，其黏附性腹膜细胞（90%至95%为巨噬细胞）在体外表现出增强的趋化性和杀肿瘤能力。这些活化的巨噬细胞在体外产生的淋巴细胞活化因子（LAF）比等量未处理小鼠的黏附细胞多2至5倍。相比之下，用巯基乙酸盐或矿物油处理的小鼠的黏附性腹膜细胞没有细胞毒性，产生的LAF也不比未处理小鼠的腹膜细胞多。未处理的裸鼠的黏附性腹膜细胞在体外具有增强的趋化性和杀肿瘤能力，与正常同窝仔鼠的黏附性腹膜细胞相比，其LAF产生也有所增强。在体外与细菌内毒素或抗原诱导的淋巴因子孵育后，黏附性腹膜细胞和巨噬细胞样肿瘤细胞系P388D1的LAF产生也明显增加。这些数据表明，黏附性腹膜细胞可在体内或体外被激活以产生更多的LAF。因此，活化的巨噬细胞在免疫反应传入支的放大及其效应功能方面比正常巨噬细胞更有效。
