Gagnon R F, MacLennan I C
Immunology. 1982 Feb;45(2):283-92.
The regulation of secondary antibody production in mice is studied in adoptive transfer experiments. Mice immunized twice with chicken red blood cells (CRBC) in Freund's complete adjuvant (adjuvant) at 28 day intervals subsequently produce high levels of IgG anti-CRBC antibody for several weeks. Immunization with CRBC in saline also induces prolonged splenic production of IgG anti-CRBC antibody but at a much lower level. It was not possible to augment the level of antibody production in mice immunized with CRBC in saline by transfer of very large numbers of spleen cells from mice immunized with CRBC in adjuvant. Transfer experiments in the opposite direction failed to suppress antibody responses in mice immunized with CRBC in adjuvant. Similar experiments were carried out with mixed transfers into third party non-immune irradiated recipients. The requirement for continued antigen supply to maintain secondary antibody production was shown in that IgG anti-CRBC antibody production in non-immune recipients of immune spleen cells ceased within five days when the recipients were not given antigen. It is concluded that: (i) while strong regulation of secondary antibody production against CRBC exists, this is not a function of transferrable suppressors; (ii) increased antibody production in animals treated with adjuvant is not due to increases in the number of antigen-sensitive T or B lymphocytes; and (iii) the data are most easily explained by a hypothesis which implicates the rate of triggering of lymphocytes as the regulatory step in secondary antibody production. This could be a factor relating to the supply of antigen or the number of sites in secondary lymphoid tissue at which antigen can trigger.
在过继转移实验中研究了小鼠二次抗体产生的调节。小鼠每隔28天用弗氏完全佐剂（佐剂）中的鸡红细胞（CRBC）免疫两次，随后数周会产生高水平的抗CRBC IgG抗体。用盐水中的CRBC免疫也会诱导脾脏长期产生抗CRBC IgG抗体，但水平要低得多。通过转移大量用佐剂中的CRBC免疫的小鼠的脾细胞，无法提高用盐水中的CRBC免疫的小鼠的抗体产生水平。相反方向的转移实验未能抑制用佐剂中的CRBC免疫的小鼠的抗体反应。对第三方非免疫照射受体进行了混合转移的类似实验。当未给予受体抗原时，免疫脾细胞的非免疫受体中的抗CRBC IgG抗体产生在五天内停止，这表明维持二次抗体产生需要持续的抗原供应。得出以下结论：（i）虽然存在针对CRBC的二次抗体产生的强调节，但这不是可转移抑制因子的功能；（ii）用佐剂处理的动物中抗体产生增加不是由于抗原敏感T或B淋巴细胞数量的增加；（iii）这些数据最容易用一个假设来解释，该假设认为淋巴细胞的触发速率是二次抗体产生中的调节步骤。这可能是一个与抗原供应或二次淋巴组织中抗原可触发的位点数量有关的因素。
