Iwakura K, Tamura H, Matsumoto A, Ajimi S, Ogura S, Kakimoto K, Matsumoto T, Hayashi M
Research Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
Mutat Res. 1992 Feb-Mar;278(2-3):131-7.
Dose-dependent induction of micronuclei with 1-beta-D-arabinofuranosylcytosine (ara-C) was clearly shown in CD-1 mouse peripheral blood reticulocytes (RETs) using an acridine orange (AO) supravital staining method, as well as in the conventional bone marrow assay. The maximum frequencies of micronucleated RETs (MNRETs) in peripheral blood and of micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow were comparable, as shown in two laboratories independently. The maximum frequencies of MNRETs in peripheral blood lagged about 24 and 12 h behind those of MNPCEs in bone marrow in experiments with 24- and 12-h sampling intervals, respectively. The proportion of each type of RET was examined periodically after treatment with ara-C at doses ranging from 6.25 to 50.0 mg/kg. The proportion of type I RETs among total RETs decreased 24 or 48 h after treatment according to the dose level. This suggest that this ratio could be a good indicator of the bone marrow cell toxicity of test chemicals.
使用吖啶橙（AO）活体染色法，在CD-1小鼠外周血网织红细胞（RETs）以及传统骨髓试验中，均清楚显示了1-β-D-阿拉伯呋喃糖基胞嘧啶（ara-C）对微核的剂量依赖性诱导作用。两个实验室独立研究表明，外周血中微核化网织红细胞（MNRETs）的最大频率与骨髓中微核化多染性红细胞（MNPCEs）的最大频率相当。在分别以24小时和12小时为采样间隔的实验中，外周血中MNRETs的最大频率分别比骨髓中MNPCEs的最大频率滞后约24小时和12小时。在用6.25至50.0 mg/kg剂量的ara-C处理后，定期检查每种类型RETs的比例。根据剂量水平，处理后24或48小时，I型RETs在总RETs中的比例下降。这表明该比率可能是测试化学品骨髓细胞毒性的良好指标。
