Kondo K, Ozawa S
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.
Mutat Res. 1992 Feb-Mar;278(2-3):109-11.
A new method for the micronucleus test using peripheral blood reticulocytes stained supravitally using acridine orange-coated slides was evaluated in male CD-1 mice treated with ethyl methanesulfonate (EMS) at doses of 100, 200, 300, and 400 mg/kg. Peripheral blood samples were taken 0, 24, 48, 72, and 96 h after treatment from each mouse without killing. The frequencies of micronucleated reticulocytes increased dose-dependently with the peak at 48 h after treatment. These results indicate that, at least for EMS, the new method used here can be an alternative to the conventional method using bone marrow polychromatic erythrocytes.
使用吖啶橙包被的玻片对经活体染色的外周血网织红细胞进行微核试验的新方法，在以100、200、300和400 mg/kg剂量的甲磺酸乙酯（EMS）处理的雄性CD-1小鼠中进行了评估。处理后0、24、48、72和96小时，在不处死每只小鼠的情况下采集外周血样本。微核化网织红细胞的频率呈剂量依赖性增加，在处理后48小时达到峰值。这些结果表明，至少对于EMS而言，此处使用的新方法可以替代使用骨髓多染性红细胞的传统方法。
