The fine localization of ATPases in cultures in vitro of chick embryo spinal cord.

A histochemical study of the ultrastructural localization of ATPases in cultures of chick embryo spinal cord has been carried out. The localization of Ca2+ and Mg2+ activated membrane ATPases appears similar: both enzyme activities are localized on the outer surfaces of plasma membranes of all kinds of cell present in the cultures, with the exception of the membranes in direct contact with the culture medium. The results are discussed in relation to data concerning the localization and function of ATPases in vivo and in relation to the possible establishment of mechanisms of nutrient uptake and transfer in cultures of nervous tissue.