Sanders E J
Cell Tissue Res. 1987 Feb;247(2):465-8. doi: 10.1007/BF00218330.
Adenylate cyclase activity was localized in various tissues of the early chick embryo using an ultrastructural histochemical technique. Reaction product was deposited on the lateral plasma membrane of all cells, but with a preferential localization at the apical terminal complex in the epiblast. There was no activity associated with the free surfaces of these or other cells in the embryo. Intracellular deposits were found in all cells associated with the endoplasmic reticulum, nuclear envelope and Golgi bodies. In the last organelle, the deposit was sometimes observed to be distributed through the stack in a non-uniform way, with the heaviest deposits occurring at the forming face. No clear difference could be detected between the cytochemical activity associated with cells in various regions of the embryo, or with embryos at different stages of early development.
采用超微结构组织化学技术，对鸡胚早期各组织中的腺苷酸环化酶活性进行了定位研究。反应产物沉积在所有细胞的外侧质膜上，但在上胚层的顶端终末复合体处有优先定位。胚胎中这些细胞或其他细胞的游离表面均未发现活性。在与内质网、核膜和高尔基体相关的所有细胞中均发现了细胞内沉积物。在最后一个细胞器中，有时观察到沉积物以不均匀的方式分布在堆叠中，最重的沉积物出现在形成面。在胚胎不同区域的细胞或早期发育不同阶段的胚胎中，相关的细胞化学活性未检测到明显差异。
