Stiemer R H, Westenfelder U, Gausepohl H, Mirshahi M, Gundt A, Frank R W, Männel D N
Laboratoire d'Immunopathologie de l'Oeil, INSERM U86, Paris, France.
J Autoimmun. 1992 Feb;5(1):15-26. doi: 10.1016/s0896-8411(05)80048-4.
A common epitope on S-antigen (arrestin), a potent autoantigen inducing experimental autoimmune uveoretinitis (EAU), and on human tumor necrosis factor alpha (hTNF alpha) was revealed using two monoclonal antibodies to S-antigen which inhibit EAU induction. The minimal common sequence for monoclonal antibody recognition is GVxLxD in the S-antigen/hTNF alpha amino acid sequences. Peptides containing this sequence motif exhibited monocyte activating capacity similar to the autocrine stimulatory capacity of hTNF alpha itself. In the S-antigen this activity was located from residue 40 to 50, corresponding to the peptide PVDGVVLVDPE (epitope S2). In hTNF alpha, the monocyte activating capacity correlated to residue 31 to 53, corresponding to the peptide RRANALLANGVELRDNQLVVPSE (peptide RRAN). The identified regions define common functional structures in the autoantigen and in the hTNF alpha molecule. The data suggest a regulatory function of this particular structure in TNF alpha expression and in autoimmunity.
利用两种抑制实验性自身免疫性葡萄膜视网膜炎（EAU）诱导的抗S抗原单克隆抗体，发现了S抗原（抑制蛋白）和人肿瘤坏死因子α（hTNFα）上的一个共同表位，S抗原是一种能诱导EAU的强效自身抗原。单克隆抗体识别的最小共同序列在S抗原/hTNFα氨基酸序列中为GVxLxD。含有该序列基序的肽表现出与hTNFα自身自分泌刺激能力相似的单核细胞激活能力。在S抗原中，该活性位于第40至50位残基，对应于肽PVDGVVLVDPE（表位S2）。在hTNFα中，单核细胞激活能力与第31至53位残基相关，对应于肽RRANALLANGVELRDNQLVVPSE（肽RRAN）。所确定的区域界定了自身抗原和hTNFα分子中的共同功能结构。数据表明该特定结构在TNFα表达和自身免疫中具有调节功能。
