单克隆抗体S2D2抑制大鼠实验性自身免疫性葡萄膜炎中针对S抗原/TNFα共同表位的体液免疫反应。

Humoral immune response against the S-antigen/TNF alpha common epitope in rat EAU suppressed by the monoclonal antibody S2D2.

作者信息

de Kozak Y, Stiemer R H, Mirshahi M, Frank R W, de Smet M, Faure J P

机构信息

Laboratoire d'Immunopathologie de l'Oeil, INSERM U 86, Paris, France.

出版信息

Curr Eye Res. 1992;11 Suppl:119-27. doi: 10.3109/02713689208999521.

DOI:10.3109/02713689208999521

PMID:1385041

Abstract

S-antigen (S-Ag)-induced experimental autoimmune uveoretinitis (EAU) in rats can be suppressed by injecting the mouse monoclonal antibody (mAb) S2D2 or a polyclonal rat anti-idiotype S2D2 (anti-Id S2D2) antibody, the internal image of the epitope of S-Ag recognized by mAb S2D2. This epitope located in amino acids 40-50 of bovine S-Ag (peptide S2), displays an homology with a sequence of human tumor necrosis factor alpha (hTNF alpha) (peptide RRAN) which is also recognized by S2D2. (Stiemer et al., this symposium). We show that one injection of S2D2 at the time of immunization with S-Ag suppressed EAU and modulated the production of antibodies against peptides of bovine or human S-Ag containing the S2 epitope and against peptide RRAN. Immunization against anti-Id S2D2 stimulated antibody production to peptide S2 and RRAN and inhibited EAU. These data suggest that disease suppression could be related to the production of antibodies against the S-Ag/TNF alpha common epitope.

摘要

在大鼠中，通过注射小鼠单克隆抗体（mAb）S2D2或多克隆大鼠抗独特型S2D2（抗Id S2D2）抗体（mAb S2D2识别的S抗原（S-Ag）表位的内影像），可抑制S抗原（S-Ag）诱导的实验性自身免疫性葡萄膜视网膜炎（EAU）。该表位位于牛S-Ag的40-50位氨基酸（肽S2），与人肿瘤坏死因子α（hTNFα）的一个序列（肽RRAN）具有同源性，且同样被S2D2识别。（Stiemer等人，本次研讨会）。我们发现，在S-Ag免疫时注射一次S2D2可抑制EAU，并调节针对含S2表位的牛或人S-Ag肽以及肽RRAN的抗体产生。针对抗Id S2D2的免疫刺激了针对肽S2和RRAN的抗体产生，并抑制了EAU。这些数据表明，疾病抑制可能与针对S-Ag/TNFα共同表位的抗体产生有关。