Bettini E, Maggi A
Milano Molecular Pharmacology Laboratory, University of Milan, Italy.
J Neurochem. 1992 May;58(5):1923-9. doi: 10.1111/j.1471-4159.1992.tb10070.x.
Differential screening of a cDNA library prepared from mRNA of the hippocampus of estrogen-stimulated ovariectomized female rats led to the identification of a single estrogen-induced clone. Analysis of the sequence identified this cDNA as the gene coding for subunit III of the enzyme cytochrome c oxidase. Cytochrome c oxidase subunit III mRNA levels significantly increased as early as 3 h following the administration of a single dose of hormone. This effect was visible in the hippocampus and in the hypothalamus, but not in the other brain areas examined. Because subunit III of the cytochrome c oxidase is of mitochondrial origin, the mechanism involved in the estrogenic effect is still unknown. The observation that the activity of cytochrome c oxidase can also be induced by estrogens in the hippocampus indicates that this induction may be secondary to the increased expression of the other subunits of cytochrome c oxidase or to the general increase of neuronal activity.
对从雌激素刺激的去卵巢雌性大鼠海马体的mRNA制备的cDNA文库进行差异筛选，导致鉴定出一个单一的雌激素诱导克隆。对该序列的分析确定这个cDNA为编码细胞色素c氧化酶亚基III的基因。在单次给予激素后3小时，细胞色素c氧化酶亚基III的mRNA水平就显著增加。这种效应在海马体和下丘脑可见，但在所检查的其他脑区未见。由于细胞色素c氧化酶亚基III起源于线粒体，雌激素效应所涉及的机制仍然未知。细胞色素c氧化酶活性也可在海马体中被雌激素诱导这一观察结果表明，这种诱导可能继发于细胞色素c氧化酶其他亚基表达的增加或神经元活性的普遍增加。
