通过DNA扩增法在尸检肺组织中检测卡氏肺孢子虫。
A search for Pneumocystis carinii in post-mortem lungs by DNA amplification.
作者信息
Peters S E, Wakefield A E, Sinclair K, Millard P R, Hopkin J M
机构信息
Department of Paediatrics, John Radcliffe Hospital, Oxford, U.K.
出版信息
J Pathol. 1992 Feb;166(2):195-8. doi: 10.1002/path.1711660217.
PMID:1373186
Abstract
DNA amplification of specific sequences and subsequent oligonucleotide hybridization were used to search for Pneumocystis carinii in post-mortem lung samplings from non-immunosuppressed individuals ranging from 15 to 70 years of age. No P. carinii-specific DNA was detected in 45 DNA amplification reactions from 15 lungs.
摘要
采用特定序列的DNA扩增及随后的寡核苷酸杂交技术，在15至70岁非免疫抑制个体的尸检肺组织样本中寻找卡氏肺孢子虫。在来自15个肺组织的45次DNA扩增反应中，未检测到卡氏肺孢子虫特异性DNA。
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