Schwonzen M, Schmits R, Baldus S E, Vierbuchen M, Hanisch F G, Pfreundschuh M, Diehl V, Bara J, Uhlenbruck G
Klinik I für Innere Medizin, Köln, Germany.
Br J Cancer. 1992 Apr;65(4):559-65. doi: 10.1038/bjc.1992.114.
Mucus glycoproteins of human amniotic fluid were used to generate a monoclonal antibody (MAb) FW6, which stained the intestine of a 24 week stage fetus. In adults, 76% of colonic adenocarcinomas (13/17) showed strong expression of the FW6 epitope, but it was not detected in the histologically normal mucosae adjacent to the tumours or in normal left colon mucosa. In addition, MAb FW6 stained large cell carcinomas of the lung (2/3), gastric carcinomas (5/11), and ovary adenocarcinomas (3/4). The expression in carcinomas can also be called ectopic for testing normal tissues. MAb FW6 was also reactive with pyloric mucus glands, Brunner's glands of the duodenum, Paneth cells of the ileum, pancreatic ducts, absorptive cells of the right colon, bronchiolar glands, kidney urothelia, and with a restricted number of normal mucinous tubuli of salivary gland. It was demonstrated to be under the control of the secretion gene only in intestinal Paneth cells and absorptive cells of the right colon. Comparative histochemical analysis comprising a panel of MAbs suggests that the corresponding epitope of the MAb FW6 is a type II chain related carbohydrate structure belonging to the Lex/Ley-antigen family, but is different from short chain Lex and Ley.
人羊水黏液糖蛋白被用于制备单克隆抗体（MAb）FW6，该抗体可对24周龄胎儿的肠道进行染色。在成年人中，76%的结肠腺癌（13/17）显示FW6表位的强表达，但在肿瘤旁组织学正常的黏膜或正常左半结肠黏膜中未检测到。此外，MAb FW6可对肺大细胞癌（2/3）、胃癌（5/11）和卵巢腺癌（3/4）进行染色。在癌组织中的表达相对于正常组织检测而言也可称为异位表达。MAb FW6还与幽门黏液腺、十二指肠的Brunner腺、回肠的潘氏细胞、胰管、右半结肠的吸收细胞、细支气管腺、肾尿路上皮以及唾液腺数量有限的正常黏液小管发生反应。结果表明，仅在肠道潘氏细胞和右半结肠的吸收细胞中，其受分泌基因的调控。由一组单克隆抗体进行的比较组织化学分析表明，MAb FW6的相应表位是一种属于Lex/Ley抗原家族的II型链相关碳水化合物结构，但不同于短链Lex和Ley。
