Hanisch F G, Egge H, Peter-Katalinić J, Uhlenbruck G, Dienst C, Fangmann R
Eur J Biochem. 1985 Oct 15;152(2):343-51. doi: 10.1111/j.1432-1033.1985.tb09204.x.
The primary structures of four major sialylated saccharide alditols derived from mucus glycoproteins of human seminal plasma were established by fast-atom-bombardment mass spectrometry and methylation analysis. Anomeric configurations of the glycosidic bonds were determined by exoglycosidase digestion and CrO3 oxidation. (Formula: see text) Two minor components represent isomers of the major saccharide in A6, which are probably characterized by terminal sequences NeuAc(2----3)Gal(1----4)[Fuc(1----3)]GlcNAc(1---- and Fuc(1----2)Gal(1----4)[NeuAc(2----6)]GlcNAc(1----, respectively. Based on quantitative data from high-pressure liquid chromatography and on structural information, the biosynthetic pathways for neutral and sialylated saccharides related to the Lewis system were proposed. Expression of saccharides A6, A7 and A8 and their asialo counterparts, which are characterized by antigenic determinants H, Lex and Ley, respectively, is qualitatively and quantitatively dependent on the Lewis blood type of the respective donor and correlates with Ca 19-9 activity of its seminal plasma.
通过快原子轰击质谱法和甲基化分析确定了源自人精浆黏液糖蛋白的四种主要唾液酸化糖醛糖醇的一级结构。糖苷键的异头构型通过外切糖苷酶消化和CrO₃氧化来确定。（分子式：见正文）两个次要成分代表A6中主要糖类的异构体，其特征可能分别为末端序列NeuAc(2→3)Gal(1→4)[Fuc(1→3)]GlcNAc(1→和Fuc(1→2)Gal(1→4)[NeuAc(2→6)]GlcNAc(1→。基于高压液相色谱的定量数据和结构信息，提出了与Lewis系统相关的中性和唾液酸化糖类的生物合成途径。糖类A6、A7和A8及其去唾液酸对应物的表达，分别以抗原决定簇H、Lex和Ley为特征，在定性和定量上取决于各自供体的Lewis血型，并与其精浆的Ca 19-9活性相关。
