Miyauchi T, Nagayama T, Maruyama K
Department of Urology, Chiba Cancer Center Hospital.
Nihon Hinyokika Gakkai Zasshi. 1992 Jan;83(1):66-74. doi: 10.5980/jpnjurol1989.83.66.
Epithelioid cells that had grown in short-term cultures derived from 10 cases of adenocarcinoma (PCa) and 10 cases of hyperplasia (BPH) of the prostate were karyotyped by the G-banding method for the pathogenesis of these disease. PCa specimens included 4 well, 2 moderately, and 4 poorly differentiated types, and were obtained by perineal needle biopsy from 4 patients in stage B and 6 patients in stage D2. Cells liberated from metastatic lymph node lesions of 2 patients with poorly differentiated PCa were also analyzed directly without cultivation in vitro. All BPH specimens were obtained by prostatectomy, and cells that had grown in epithelioid pattern in short-term cultures were analyzed. In PCa, hyperploidy was seen in all but 2 cases. Structure analysis disclosed abnormality of chromosome 16 in 4 PCa, deletion of Y in 3 PCa, abnormality of chromosomes 7, 14, 15, 18, and 19 in 2 PCa, and abnormality of chromosomes 3, 4, 17, and 21 in 1 PCa. Multiple markers were observed in 1 patient, and hyperploidy in another patient with metastatic lymph nodes. All but 2 cases of BPH were diploid. Normal male karyotypes were seen in 6 BPH. Trisomy of chromosomes 7 and 16 were observed in 2 BPH. Of 4 patients with stage B PCa, 3 who have been alive for 3 years to date had multiple abnormalities, whereas 1 patient who died 2 years after diagnosis had few abnormalities.(ABSTRACT TRUNCATED AT 250 WORDS)
采用G显带法对来自10例前列腺腺癌（PCa）和10例前列腺增生（BPH）的短期培养物中生长的上皮样细胞进行核型分析，以探讨这些疾病的发病机制。PCa标本包括4例高分化、2例中分化和4例低分化类型，通过会阴针吸活检从4例B期和6例D2期患者获取。对2例低分化PCa患者转移淋巴结病变中直接获取的未体外培养细胞也进行了分析。所有BPH标本均通过前列腺切除术获得，并对短期培养中呈上皮样生长模式的细胞进行分析。在PCa中，除2例之外均可见超二倍体。结构分析显示，4例PCa存在16号染色体异常，3例PCa存在Y染色体缺失，2例PCa存在7、14、15、18和19号染色体异常，1例PCa存在3、4、17和21号染色体异常。1例患者观察到多个标记，另1例有转移淋巴结的患者存在超二倍体。除2例BPH外均为二倍体。6例BPH可见正常男性核型。2例BPH观察到7号和16号染色体三体。4例B期PCa患者中，3例至今存活3年有多个异常，而1例诊断后2年死亡的患者异常较少。（摘要截短于250字）
