Zhang Longyang, Guo Feng, Gao Xinghua, Wu Yanlin
Department of Urology, Jinan Central Hospital of Shandong University, Jinan, Shangdong 250013, P.R. China.
Mol Med Rep. 2015 Jul;12(1):1298-304. doi: 10.3892/mmr.2015.3455. Epub 2015 Mar 9.
Golgi phosphoprotein 3 (GOLPH3) has recently been implicated as an oncogene involved in the development of carcinoma in a number of organs. The expression of GOLPH3 in prostate cancer (PCa) tissues was investigated in the present study. Human PC-3 and LNCaP PCa cell lines were analyzed in order to assess whether silencing of GOLPH3 expression affects cell vitality, migration and invasion, in vitro. An immunohistochemistry analysis was performed in order to measure the expression of GOLPH3 in samples from 117 patients with PCa and from 50 patients with benign prostatic hyperplasia (BPH). Associations between GOLPH3 expression and clinicopathological parameters, such as overall survival, were assessed. GOLPH3 expression was shown to be significantly greater in PCa tissues than in BPH tissues. GOLPH3 expression was positively correlated with Gleason score (P=0.031), tumor stage (T stage; P=0.020) and lymph node status (P=0.013), in patients with PCa. Biochemical recurrence-free survival (serum prostate-specific antigen-based) and overall survival, were reduced in patients with GOLPH3-positive PCa. A multivariate analysis indicated that GOLPH3 expression was an independent predictor of biochemical recurrence-free survival [hazard ratio (HR), 2.943; 95% confidence interval (CI), 1.190-5.521; P=0.028], and of overall survival (HR, 4.371; 95% CI, 2.045-7.109; P=0.014). Transfection with GOLPH3‑targeted small interfering RNA reduced the capability of PC-3 and LNCaP cell lines to proliferate, migrate and invade in vitro, compared with the controls. The level of GOLPH3 expression in radical prostatectomy samples may be useful for predicting biochemical recurrence-free survival and overall survival in patients with PCa.
高尔基体磷蛋白3（GOLPH3）最近被认为是一种癌基因，参与多种器官癌症的发生发展。本研究调查了GOLPH3在前列腺癌（PCa）组织中的表达情况。分析了人PC-3和LNCaP前列腺癌细胞系，以评估体外沉默GOLPH3表达是否会影响细胞活力、迁移和侵袭。进行免疫组织化学分析，以检测117例前列腺癌患者和50例良性前列腺增生（BPH）患者样本中GOLPH3的表达。评估了GOLPH3表达与临床病理参数（如总生存期）之间的关联。结果显示，PCa组织中GOLPH3的表达明显高于BPH组织。在前列腺癌患者中，GOLPH3表达与Gleason评分（P=0.031）、肿瘤分期（T分期；P=0.020）和淋巴结状态（P=0.013）呈正相关。GOLPH3阳性前列腺癌患者的无生化复发生存期（基于血清前列腺特异性抗原）和总生存期缩短。多因素分析表明，GOLPH3表达是无生化复发生存期的独立预测因子[风险比（HR），2.943；95%置信区间（CI），1.190 - 5.521；P=0.028]，也是总生存期的独立预测因子（HR，4.371；95% CI，2.045 - 7.109；P=0.014）。与对照组相比，用靶向GOLPH3的小干扰RNA转染可降低PC-3和LNCaP细胞系在体外的增殖、迁移和侵袭能力。根治性前列腺切除术样本中GOLPH3的表达水平可能有助于预测前列腺癌患者的无生化复发生存期和总生存期。
