Benigni R, Giuliani A
Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di Sanità, Rome, Italy.
Mutat Res. 1992 Apr;266(2):71-6. doi: 10.1016/0027-5107(92)90173-y.
A great deal of information on short-term mutagenicity assays presently exists, having been generated through individual as well as large comparative programs. The comparative programs have often examined the same tests, but with different sets of chemicals; this then gives rise to the problem of how to identify the information which is common to the different data bases, i.e., the general properties of the assays. This paper continues previous analyses of this subject, and describes a general approach by which different and heterogeneous data bases can be compared to each other. The results relative to 4 assays (Salmonella typhimurium gene mutation, mouse lymphoma L5178Y cell gene mutation, chromosomal aberrations in CHO cells, and SCEs in CHO cells) in 4 different data bases were studied. Factor analysis was used to model the different pieces of information. The analysis demonstrated a concordance between the indications of the U.S. National Toxicology Program and the International Program for the Evaluation of Short-Term Tests for Carcinogens, whereas the results of Gene-Tox and the International Program for Chemical Safety turned out to be biased, to different degrees, by their specific aims and characteristics. Moreover, the general properties--independent of the specific data bases--of the 4 assays were highlighted, and the similarities between the performances of the assays were given a quantitative measure.
目前存在大量关于短期致突变性试验的信息，这些信息是通过个别项目以及大型比较项目产生的。比较项目常常对相同的试验进行检验，但使用的化学物质不同；这就产生了一个问题，即如何识别不同数据库中共同的信息，也就是这些试验的一般特性。本文延续了此前对该主题的分析，并描述了一种可用于相互比较不同且异质数据库的通用方法。研究了4个不同数据库中与4种试验（鼠伤寒沙门氏菌基因突变、小鼠淋巴瘤L5178Y细胞基因突变、中国仓鼠卵巢细胞染色体畸变以及中国仓鼠卵巢细胞姐妹染色单体交换）相关的结果。因子分析被用于对不同的信息片段进行建模。分析表明，美国国家毒理学计划和国际致癌物短期试验评估计划的指示结果具有一致性，而基因毒性计划和国际化学品安全计划的结果在不同程度上因其特定目标和特点而存在偏差。此外，突出了这4种试验独立于特定数据库的一般特性，并对这些试验性能之间的相似性给出了定量衡量。
