Pérez-Sánchez R, Oleaga-Pérez A, Encinas-Grandes A
Department of Parisitology, Faculty of Pharmacy, Salamanca University, Spain.
Parasite Immunol. 1992 Mar;14(2):201-16. doi: 10.1111/j.1365-3024.1992.tb00461.x.
In Spain, considerable efforts are currently being devoted to the eradication of Ornithodoros erraticus from the swine farms harbouring this parasite, the European vector of African swine fever (ASF). However, to do so, a preliminary requirement is to determine on which farms it is present. Of all possible methods for discovering this, the only one feasible for large scale application is the serological detection of swine bearing anti-O. erraticus antibodies. To apply serology it was necessary to check the specificity of extracts from the salivary glands (SGE) from O. erraticus. For this, indirect ELISA, competitive ELISA and Western blot were used to assay the SGE from O. erraticus and their corresponding antisera against the SGE and respective antisera from 4 ixodidae, one mange mite, one louse and a mosquito. The results obtained show that only the anti-ixodidae sera are able to react against the SGE from O. erraticus. The cause of this reaction are the somatic antigens present in the SGE of the argasid but not its soluble (secretory) antigens. It is proposed that the anti-cement antibodies present in the anti-ixodidae sera are those that react with the somatic antigens of O. erraticus.
在西班牙，目前正投入大量努力，试图在携带非洲猪瘟（ASF）欧洲传播媒介——游走钝缘蜱的养猪场中根除这种寄生虫。然而，要做到这一点，首要条件是确定哪些农场存在这种蜱虫。在所有可能发现蜱虫的方法中，唯一适用于大规模应用的是对携带抗游走钝缘蜱抗体的猪进行血清学检测。为了应用血清学方法，有必要检测游走钝缘蜱唾液腺提取物（SGE）的特异性。为此，使用间接酶联免疫吸附测定（ELISA）、竞争ELISA和蛋白质印迹法，检测游走钝缘蜱的SGE及其相应抗血清，以及来自4种硬蜱科蜱虫、1种疥螨、1种虱子和1种蚊子的相应抗血清。所得结果表明，只有抗硬蜱科蜱虫血清能够与游走钝缘蜱的SGE发生反应。这种反应的原因是钝缘蜱科蜱虫SGE中存在的体细胞抗原，而非其可溶性（分泌性）抗原。有人提出，抗硬蜱科蜱虫血清中存在的抗黏合剂抗体是与游走钝缘蜱体细胞抗原发生反应的抗体。
