Baranda J A, Pérez-Sánchez R, Oleaga-Pérez A, Encinas-Grandes A
Laboratorio of Parasitología, Facultad de Farmacia, Universidad de Salamanca, Spain.
J Parasitol. 1997 Oct;83(5):831-8.
We show by sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blot that the composition of the soluble extracts of salivary glands (SGE-2) of Ornithodoros erraticus and Ornithodoros moubata is similar to that of the saliva (pilocarpine-induced), and that the extracts are a valid source of antigens for the detection of anti-argasid antibodies. It is also shown that the SGE-2s do not vary qualitatively with the developmental stage, physiological status, or sex of the ticks. The antigenic components (at least in O. erraticus) are released into the SGE-2 by the action of enzymes that can be inhibited by phenylmethane sulfonyl fluoride plus EDTA. Most of the components of the SGE-2, except the antigenic ones, are strongly glycosylated. Accordingly, the deglycosylation of the SGE-2s does not affect the recognition of antigenic components by anti-tick sera. In both species, the major components of the SGE-2s or the saliva are not recognized by the corresponding antisera. These nonimmunogenic components could have vaccinal value but not diagnostic interest. Finally, it is shown that the antigens of O. erraticus and O. moubata do not cross-react with one another and that those of the first species are more antigenic than those of the second.
我们通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳和蛋白质免疫印迹法表明，多变钝缘蜱和莫氏钝缘蜱唾液腺可溶性提取物（SGE - 2）的成分与毛果芸香碱诱导的唾液成分相似，且这些提取物是检测抗钝缘蜱抗体的有效抗原来源。还表明，SGE - 2在蜱的发育阶段、生理状态或性别方面没有定性差异。抗原成分（至少在多变钝缘蜱中）通过可被苯甲基磺酰氟加乙二胺四乙酸抑制的酶的作用释放到SGE - 2中。SGE - 2的大多数成分，除了抗原成分外，都有强烈的糖基化。因此，SGE - 2的去糖基化并不影响抗蜱血清对抗原成分的识别。在这两个物种中，SGE - 2或唾液的主要成分都不能被相应的抗血清识别。这些非免疫原性成分可能具有疫苗价值，但对诊断没有意义。最后，表明多变钝缘蜱和莫氏钝缘蜱的抗原不会相互交叉反应，且第一个物种的抗原比第二个物种的抗原性更强。
