Canals A, Oleaga A, Pérez R, Domínguez J, Encinas A, Sánchez-Vizcaíno J M
Departamento de Sanidad Animal, INIA, Madrid, Spain.
Vet Parasitol. 1990 Oct;37(2):145-53. doi: 10.1016/0304-4017(90)90069-n.
Ornithodoros erraticus is known to transmit the virus that causes the highly contagious disease, African Swine Fever, in Spain. As part of the disease eradication campaign, an ELISA test to detect specific antibodies against the tick was developed. The ELISA, using salivary gland preparations as an antigen, showed high sensitivity and was able to detect as few as 10 adult ticks. The specific antibodies were detected in the sera 6 weeks after the primary infestation and strongly increased after the challenge. The utility of this test under field conditions was also tested.
已知西班牙的扇头蜱会传播导致高传染性疾病——非洲猪瘟的病毒。作为疾病根除行动的一部分，研发了一种用于检测针对该蜱虫的特异性抗体的酶联免疫吸附测定（ELISA）试验。该ELISA试验以唾液腺制剂作为抗原，显示出高灵敏度，能够检测低至10只成年蜱虫。在初次感染后6周能在血清中检测到特异性抗体，在再次感染后抗体水平大幅上升。还测试了该试验在野外条件下的实用性。
