编码哺乳动物剪接因子SC35的互补DNA的分离。

Isolation of a complementary DNA that encodes the mammalian splicing factor SC35.

作者信息

Fu X D, Maniatis T

机构信息

Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.

出版信息

Science. 1992 Apr 24;256(5056):535-8. doi: 10.1126/science.1373910.

DOI:10.1126/science.1373910

PMID:1373910

Abstract

The mammalian splicing factor SC35 is required for the first step in the splicing reaction and for spliceosome assembly. The cloning and characterization of a complementary DNA encoding this protein revealed that it is a member of a family of splicing factors that includes mammalian SF2/ASF. This family of proteins is characterized by the presence of a ribonucleoprotein (RNP)-type RNA binding motif and a carboxyl-terminal serine-arginine-rich (SR) domain. A search of the DNA sequence database revealed that the thymus-specific exon (ET) of the c-myb proto-oncogene is encoded on the antisense strand of the SC35 gene.

摘要

哺乳动物剪接因子SC35是剪接反应第一步和剪接体组装所必需的。编码该蛋白的互补DNA的克隆和特性分析表明，它是一个剪接因子家族的成员，该家族包括哺乳动物SF2/ASF。这类蛋白质的特征是存在核糖核蛋白（RNP）型RNA结合基序和富含丝氨酸-精氨酸的羧基末端（SR）结构域。对DNA序列数据库的搜索显示，c-myb原癌基因的胸腺特异性外显子（ET）编码在SC35基因的反义链上。