Pabst R, Reynolds J D
J Immunol. 1986 Mar 15;136(6):2011-7.
The emigration of newly produced lymphocytes from Peyer's patches (PP) of lambs was studied. Mesenteric lymph nodes (MLN) were excised from most animals a few weeks after birth, and then at 8 to 10 wk of age, the dividing cells in 3 to 4 m of the small intestine were labeled in situ with [3H]thymidine. An extracorporeal perfusion system was used to restrict the 15-min period of labeling to the perfused lengths of intestine, which included either the large continuous ileal PP or a number of smaller jejunal PP. One or 3 days later, the number of labeled cells in the perfused tissue and in other lymphoid organs was studied by autoradiography. In the perfused tissues, labeled lymphocytes accounted for 63.7% of ileal PP cells by 1 day and for 86.7% by 3 days compared with only 9.6% of lymphocytes in the perfused MLN. Labeled lymphoid cells in the perfused PP were nearly all in the follicles. Labeled lymphocytes that must have been produced in the segments of ileum or jejunum at the time of the perfusion, subsequently emigrated via the lymphatics, and were identified in the spleen, MLN, other lymph nodes, blood, jejunal PP, and at a lower frequency in the thymus, nonperfused ileal PP, and bone marrow. In lymph nodes, spleen, and nonperfused PP, more than 80% of the immigrant newly formed PP-derived cells were small- and medium-sized lymphocytes, and about 15% were large lymphocytes. The nature of the labeled cells in the lamina propria of the nonperfused small intestine was quite different in that approximately 50% were plasma cells as early as 24 hr after the cells were born in the perfused gut. It is proposed that terminal B cell differentiation was most likely initiated within the PP in response to the entry of antigen. It was estimated that at both 1 and 3 days after perfusion there were about 100 times more labeled cells in the perfused ileal PP than could be accounted for by emigration to other organs. It was concluded that these results provide additional support for the view that PP in lambs produce a tremendous number of lymphocytes, but relatively few leave their site of production; most apparently die in situ.
对新生羔羊派伊尔氏结（PP）中新产生的淋巴细胞的迁移进行了研究。大多数动物在出生后几周切除肠系膜淋巴结（MLN），然后在8至10周龄时，用[3H]胸腺嘧啶核苷对小肠3至4米处的分裂细胞进行原位标记。使用体外灌注系统将标记的15分钟时间段限制在灌注的肠段长度内，该肠段长度包括连续的大段回肠PP或多个较小的空肠PP。1天或3天后，通过放射自显影研究灌注组织和其他淋巴器官中标记细胞的数量。在灌注组织中，与灌注的MLN中仅9.6%的淋巴细胞相比，标记的淋巴细胞在1天时占回肠PP细胞的63.7%，在3天时占86.7%。灌注的PP中标记的淋巴细胞几乎都在滤泡中。在灌注时必定在回肠或空肠段产生的标记淋巴细胞，随后通过淋巴管迁移，并在脾脏、MLN、其他淋巴结、血液、空肠PP中被识别，在胸腺、未灌注的回肠PP和骨髓中出现的频率较低。在淋巴结、脾脏和未灌注的PP中，超过80%的源自PP的新形成的迁移细胞是中小型淋巴细胞，约15%是大型淋巴细胞。未灌注的小肠固有层中标记细胞的性质有很大不同，早在细胞在灌注肠道中产生后24小时，约50%是浆细胞。有人提出，终末B细胞分化最有可能在PP内对抗原的进入作出反应时启动。据估计，在灌注后1天和3天，灌注的回肠PP中标记细胞的数量比迁移到其他器官的数量多约100倍。得出的结论是，这些结果为以下观点提供了更多支持：羔羊的PP产生大量淋巴细胞，但相对较少的淋巴细胞离开其产生部位；大多数显然在原位死亡。
