Belai A, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
Brain Res. 1992 Mar 20;575(2):356-8. doi: 10.1016/0006-8993(92)90105-i.
Treatment of segments of ileum from 8-week streptozotocin-induced diabetic rats with phorbol 12,13-dibutyrate (PDBu) in vitro resulted in restoration of the diabetes-induced changes in the expression of enteric VIP- and galanin-like immunoreactive nerve fibres. The increase in fluorescence intensity and density of VIP- and galanin-like immunoreactivity observed in 8-week streptozotocin-treated rats was reduced to a near normal level after 40 min incubation of diabetic tissues in Krebs solution containing PDBu (100 nM). The tissue content of VIP was also affected (control = 2.1 +/- 0.31 pmol/cm; diabetic = 4.6 +/- 0.48 pmol/cm; diabetic + PDBu = 2.9 +/- 0.91 pmol/cm) after incubation with PDBu. The significance of these findings in relation to the possible role of protein kinase C in the regulation of expression and/or storage of these enteric neuropeptides in normal and diabetic states is discussed.
用佛波醇12,13 - 二丁酸酯（PDBu）体外处理8周链脲佐菌素诱导的糖尿病大鼠的回肠段，可使糖尿病引起的肠内血管活性肠肽（VIP）和甘丙肽样免疫反应性神经纤维表达变化恢复正常。在含有PDBu（100 nM）的Krebs溶液中孵育糖尿病组织40分钟后，8周链脲佐菌素处理大鼠中观察到的VIP和甘丙肽样免疫反应性荧光强度和密度增加降低至接近正常水平。孵育PDBu后，VIP的组织含量也受到影响（对照 = 2.1±0.31 pmol/cm；糖尿病 = 4.6±0.48 pmol/cm；糖尿病 + PDBu = 2.9±0.91 pmol/cm）。本文讨论了这些发现对于蛋白激酶C在正常和糖尿病状态下调节这些肠神经肽表达和/或储存中可能作用的意义。
