Matthews C J, Redfern C P, Hirst B H, Thomas E J
Department of Physiological Sciences, University of Newcastle upon Tyne, United Kingdom.
Fertil Steril. 1992 May;57(5):990-7. doi: 10.1016/s0015-0282(16)55014-6.
To initiate in vitro cultures of separate stromal and epithelial elements from endometriotic tissue and to compare the characteristics of these cells with those of cultured endometrial cells.
The study involved testing the viability of a culture system for endometriotic tissue and examination of the phenotype of the cells.
Fresh tissue samples were collected from the operating theater and transferred to the tissue culture laboratory.
PATIENTS, PARTICIPANTS: Twenty patients undergoing laparotomy for endometriosis and patients undergoing surgery for benign conditions were recruited.
Endometrium and endometriotic tissue were separated, cultured in vitro, and labeled by indirect immunofluorescence with monoclonal antibodies against cytoskeletal components and epithelial mucins.
Endometriotic cells have been maintained in vitro and found to resemble endometrial cells closely.
With respect to the staining patterns for cytokeratins 18 and 19, vimentin, and three different epithelial mucins, cultured cells from both endometrium and endometriotic tissue had similar properties. Cytokeratins were located in epithelial cells, and vimentin was expressed in both stromal and epithelial cells. The antimucin antibodies all gave distinct patterns of intracellular staining of epithelial cells.
Our results indicate a close similarity between cultured stromal and epithelial cells from endometrium and endometriotic deposits. Culture of these cell populations will permit study of their properties and interactions and may provide some insight into the cause of endometriosis.
从子宫内膜异位组织中分离出基质和上皮成分进行体外培养，并将这些细胞的特征与培养的子宫内膜细胞的特征进行比较。
该研究包括测试子宫内膜异位组织培养系统的活力以及检查细胞的表型。
从手术室收集新鲜组织样本并转移到组织培养实验室。
患者、参与者：招募了20例因子宫内膜异位症接受剖腹手术的患者和因良性疾病接受手术的患者。
将子宫内膜和子宫内膜异位组织分离、体外培养，并用针对细胞骨架成分和上皮粘蛋白的单克隆抗体进行间接免疫荧光标记。
子宫内膜异位细胞已在体外培养，并发现与子宫内膜细胞非常相似。
就细胞角蛋白18和19、波形蛋白以及三种不同上皮粘蛋白的染色模式而言，来自子宫内膜和子宫内膜异位组织的培养细胞具有相似的特性。细胞角蛋白位于上皮细胞中，波形蛋白在基质细胞和上皮细胞中均有表达。抗粘蛋白抗体均给出上皮细胞胞内染色的独特模式。
我们的结果表明，来自子宫内膜和子宫内膜异位沉积物的培养基质细胞和上皮细胞之间存在密切相似性。这些细胞群体的培养将有助于研究它们的特性和相互作用，并可能为子宫内膜异位症的病因提供一些见解。
