Sharpe K L, Zimmer R L, Griffin W T, Penney L L
Department of Obstetrics and Gynecology, University of Missouri, Columbia.
Fertil Steril. 1993 Nov;60(5):839-51. doi: 10.1016/s0015-0282(16)56285-2.
To identify and compare the pattern of polypeptide synthesis and release of endometriosis with that of the normal endometrium in culture.
Endometriosis and endometrial biopsy specimens were obtained from women presenting for a variety of diagnostic and therapeutic examinations. Specimens were cultured as isolated epithelial and stromal cells and tissue explants with L-[35S]methionine. De novo synthesized proteins were identified by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis, fluorography, and Western blot analysis.
Five major deviations in protein synthesis and secretion were noted when comparing endometriotic and endometrial culture media. Endometriosis synthesized and secreted two proteins of stromal cell origin that were not produced by normal endometrium: endometriosis protein group I (M(r) 40,000 to 55,000; pI 4.0 to 5.2) and endometriosis protein group II (M(r) 30,000 to 32,000; pI 7.0 to 9.0). Conversely, endometriosis lacked the ability to secrete or asynchronously secreted three groups of secretory phase endometrial proteins in culture: endometrial protein group I (M(r) 25,000 to 27,000; pI 4.5 to 5.5) and endometrial protein group II (M(r) 68,000 to 72,000; pI 3.0 to 3.2) were endometrial epithelial cell products whereas endometrial protein group III (M(r) 70,000; pI 5.7) was of endometrial stromal cell origin.
Unique characteristics in endometriosis protein synthesis and secretion as compared with the endometrium indicate biochemical dissimilarities between these tissues, which may be used to develop diagnostic markers and gain insight into the etiology and/or pathophysiology of the disease.
鉴定并比较子宫内膜异位症与正常子宫内膜在培养条件下多肽合成及释放的模式。
从因各种诊断和治疗检查前来就诊的女性身上获取子宫内膜异位症和子宫内膜活检标本。将标本作为分离的上皮细胞、基质细胞及组织外植体，加入L-[35S]甲硫氨酸进行培养。通过二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、荧光显影及蛋白质印迹分析鉴定新生合成的蛋白质。
比较子宫内膜异位症和子宫内膜的培养基时，发现蛋白质合成和分泌存在五个主要差异。子宫内膜异位症合成并分泌了两种正常子宫内膜不产生的基质细胞源性蛋白质：子宫内膜异位症蛋白组I（相对分子质量40,000至55,000；等电点4.0至5.2）和子宫内膜异位症蛋白组II（相对分子质量30,000至32,000；等电点7.0至9.0）。相反，子宫内膜异位症在培养中缺乏分泌或异步分泌三组分泌期子宫内膜蛋白质的能力：子宫内膜蛋白组I（相对分子质量25,000至27,000；等电点4.5至5.5）和子宫内膜蛋白组II（相对分子质量68,000至72,000；等电点3.0至3.2）是子宫内膜上皮细胞产物，而子宫内膜蛋白组III（相对分子质量70,000；等电点5.7）是子宫内膜基质细胞源性产物。
与子宫内膜相比，子宫内膜异位症蛋白质合成和分泌的独特特征表明这些组织之间存在生化差异，这可用于开发诊断标志物并深入了解该疾病的病因和/或病理生理学。
