Rooney I A, Davies A, Morgan B P
Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, U.K.
Immunology. 1992 Mar;75(3):499-506.
Although antibody and complement are known to cause immobilization and killing of spermatozoa in vitro the components of the complement system mediating these effects remain undefined. Here we have examined the effects of the membrane attack complex (MAC) on spermatozoa and demonstrate that spermatotoxic effects are dependent on assembly of the complete MAC. We subsequently examined the presence and functional significance of the complement regulatory proteins decay accelerating factor (DAF), MAC-inhibiting protein (MIP) and CD59 antigen on spermatozoa. Both DAF and CD59 antigen were present on the membranes of these cells. Neutralization of CD59 antigen with specific antibodies increased the susceptibility of the cells to MAC-mediated damage, suggesting a role for this molecule in the protection of spermatozoa from complement-mediated damage in the female reproductive tract.
虽然已知抗体和补体在体外可导致精子制动和死亡，但介导这些效应的补体系统成分仍不明确。在此，我们研究了膜攻击复合物（MAC）对精子的影响，并证明精子毒性效应取决于完整MAC的组装。随后，我们检测了补体调节蛋白衰变加速因子（DAF）、MAC抑制蛋白（MIP）和CD59抗原在精子上的存在情况及其功能意义。DAF和CD59抗原均存在于这些细胞的膜上。用特异性抗体中和CD59抗原会增加细胞对MAC介导损伤的敏感性，表明该分子在保护精子免受女性生殖道中补体介导损伤方面发挥作用。
