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保护人羊膜上皮细胞（HAEC）免受补体介导的溶解：三种补体抑制膜蛋白在细胞上的表达。

Protection of human amniotic epithelial cells (HAEC) from complement-mediated lysis: expression on the cells of three complement inhibitory membrane proteins.

作者信息

Rooney I A, Morgan B P

机构信息

Department of Medical Biochemistry, UWCM, Heath Park, Cardiff, U.K.

出版信息

Immunology. 1990 Nov;71(3):308-11.

PMID:1702747
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1384423/
Abstract

Cultured human amniotic epithelial cells (HAEC) were found by immunofluorescence microscopy to express three complement inhibitory membrane proteins, CD59 antigen, decay-accelerating factor (DAF) and membrane attack complex (MAC) inhibitory protein (MIP), on their surfaces. The effects of incubation with Fab2 fragments of monoclonal antibodies (mAb) raised against these proteins on susceptibility of sensitized cells to lysis by homologous complement was examined. Percentage cell lysis was markedly increased in the presence of Fab2 anti-CD59 and to a lesser, but significant, extent in the presence of Fab2 anti-DAF. Fab2 anti-MIP did not alter the sensitivity of the cells to lysis by complement.

摘要

通过免疫荧光显微镜检查发现，培养的人羊膜上皮细胞（HAEC）在其表面表达三种补体抑制膜蛋白，即CD59抗原、衰变加速因子（DAF）和膜攻击复合物（MAC）抑制蛋白（MIP）。研究了用针对这些蛋白产生的单克隆抗体（mAb）的Fab2片段孵育对致敏细胞对同源补体裂解敏感性的影响。在存在Fab2抗CD59的情况下，细胞裂解百分比显著增加，在存在Fab2抗DAF的情况下，细胞裂解百分比虽增加程度较小但也显著。Fab2抗MIP并未改变细胞对补体裂解的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c5/1384423/dd47b110e75b/immunology00126-0005-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c5/1384423/dd47b110e75b/immunology00126-0005-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53c5/1384423/dd47b110e75b/immunology00126-0005-a.jpg

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The Immunomodulatory Properties of Amniotic Cells: The Two Sides of the Coin.羊膜细胞的免疫调节特性：硬币的两面。
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Both freshly prepared and frozen-stored amniotic membrane cells express the complement inhibitor CD59.新鲜制备和冷冻保存的羊膜细胞均表达补体抑制剂CD59。

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Isolation and characterization of the complement-inhibiting protein CD59 antigen from platelet membranes.从血小板膜中分离和鉴定补体抑制蛋白CD59抗原
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J Immunol. 1982 Jul;129(1):184-9.
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The formation of HL-A antibodies in pregnancy. The antigenicity of aborted and term fetuses.孕期HL - A抗体的形成。流产胎儿与足月胎儿的抗原性。
J Obstet Gynaecol Br Commonw. 1974 Mar;81(3):222-8. doi: 10.1111/j.1471-0528.1974.tb00449.x.
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A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity.一种用于在细胞毒性测量和肿瘤坏死因子（TNF）活性测定中定量乳酸脱氢酶释放的快速简便方法。
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