REVEL J P, NAPOLITANO L, FAWCETT D W
J Biophys Biochem Cytol. 1960 Dec;8(3):575-89. doi: 10.1083/jcb.8.3.575.
The electron microscopic appearance of glycogen has been studied in the organs of several animal species. Glycogen almost always appears as roughly circular granules from 150 to 400 A in diameter. The intrinsic electron density of glycogen varies from tissue to tissue; however, treatment with lead hydroxide as described by Watson deeply stains the granules. Glycogen pellets were isolated from some of the tissues studied by centrifugation. Such pellets were shown to be glycogen by chemical and histochemical criteria. When thin sections of the pellet are examined under the electron microscope they can be seen to consist of densely packed granules similar to those found in the intact tissues. Such pellets are also stained for electron microscopy by short exposure to lead hydroxide.
已对几种动物物种器官中糖原的电子显微镜外观进行了研究。糖原几乎总是呈现为直径150至400埃的大致圆形颗粒。糖原的固有电子密度因组织而异；然而，按照沃森所述用氢氧化铅处理会使颗粒深度染色。通过离心从一些研究的组织中分离出糖原沉淀。通过化学和组织化学标准证明这些沉淀是糖原。当在电子显微镜下检查沉淀的薄片时，可以看到它们由紧密堆积的颗粒组成，类似于在完整组织中发现的颗粒。通过短时间暴露于氢氧化铅，这些沉淀也可用于电子显微镜染色。
