Galli A, Della Croce C, Minnucci S, Fiorio R, Bronzetti G
Istituto di Mutagenesi e Differenziamento CNR, Pisa, Italy.
Mutat Res. 1992 May;282(1):55-60. doi: 10.1016/0165-7992(92)90074-r.
The activity of cinnamaldehyde (CIN), a bioantimutagen in bacterial systems, was tested in the D7 strain of yeast Saccharomyces cerevisiae. Yeast cells were UV-irradiated and post-incubated in liquid growth medium for 2 and 4 h with different concentrations of cinnamaldehyde. During the post-incubation period, DNA-damage-specific functions may be induced. This in turn may affect the genotoxicity and in fact a weak decrease in UV-induced convertant and revertant frequencies was observed after 4 h of post-incubation. The presence of CIN in the growth medium increased the UV-induced gene conversion and reversion. The addition of cycloheximide abolished this effect. To evaluate the CIN effect on protein synthesis, extracts of cells UV-treated and post-incubated for 2 h in the presence of 35S-methionine were performed. SDS-gel electrophoresis demonstrated the inhibitory effect of CIN on a UV-specific protein. This work suggests that CIN might interfere with DNA-damage-inducible systems although it did not exert an antimutagenic activity in our experimental conditions.
在酿酒酵母D7菌株中测试了肉桂醛（CIN）的活性，它在细菌系统中是一种生物抗诱变剂。酵母细胞经紫外线照射后，在液体生长培养基中与不同浓度的肉桂醛一起后培养2小时和4小时。在后培养期间，可能会诱导DNA损伤特异性功能。这反过来可能会影响遗传毒性，事实上，在后培养4小时后，观察到紫外线诱导的转化体和回复体频率略有下降。生长培养基中CIN的存在增加了紫外线诱导的基因转化和回复。添加环己酰亚胺消除了这种效应。为了评估CIN对蛋白质合成的影响，对在35S-甲硫氨酸存在下经紫外线处理并后培养2小时的细胞提取物进行了研究。SDS-凝胶电泳证明了CIN对一种紫外线特异性蛋白质的抑制作用。这项工作表明，CIN可能会干扰DNA损伤诱导系统，尽管在我们的实验条件下它没有发挥抗诱变活性。
